Malus micromalus makino tissue culture method

Abstract

The present invention discloses a malus micromalus makino tissue culture method, which comprises: selecting malus micromalus makino with good growth as a material, taking the sterile blade cut block with a length of 0.5 cm and a width of 0.5 cm, the leaf stalk with a length of about 1 cm, and the stem section long strip, and uniformly inoculating into a culture medium a; after about 5 weeks, forming significant cluster buds, cutting the cluster buds into small blocks, respectively inoculating into culture mediums b and c, and proliferating; after four weeks, when the cut block enters a mass proliferation stage, carrying out transfer inoculation of the differentiated bud into a culture medium d, and culturing; after about 3 weeks, inoculating the root-free-like seedling with a height of about 3 cm and strong growth into a culture medium e; after 3 weeks, growing white fibrous roots on the base portion of the seedling; and placing the test-tube plantlet in a ventilation place for 3 days, taking out the small seedling, transplanting into a mixing matrix, and continuously culturing. With the method of the present invention, a large number of the high quality malus micromalus makino seedlings can be bred, and the survival rate is more than or equal to 95%.

Description

technical field [0001] The invention relates to the technical field of crabapple culture, in particular to a method for tissue culture of crabapple in Xifu. Background technique [0002] Xifu crabapple, also known as small fruit crabapple, is a plant of the genus Apple in the Rosaceae family. It was named after it grew in Xifu during the Jin Dynasty. Branches are erect, up to 2.5-5 meters. Branchlets are thin and cylindrical, pubescent when young, shedding when old, purplish red or dark brown, with sparse lenticels. Its flowers are in an umbel-shaped raceme, with 4-7 flowers gathered on the top of the branchlets, and the pedicels are 2-3 cm long. Its flowering period is from April to May, and the fruiting period is from August to September. [0003] Xifu crabapple stands tall among the crabapple flowers, like a slim girl. The flowers are red, the leaves are green, and the fruit is beautiful, regardless of whether it is planted alone, in a row or in a cluster, it is very...

AI Technical Summary

Problems solved by technology

[0004] In addition, the commonly used propagation methods of Xifu crabapple are usually grafting and division propagation, and methods such as sowing, layering and root cuttings can also be used for propagation. However, the quality of seedlings grown by these two traditional propagation methods is not high, and The reproductive coefficient is low, which leads to a shortage in the market