Malus micromalus makino tissue culture method

A tissue culture, crabapple technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low quality of seedlings, short supply, low reproduction coefficient, etc.

Inactive Publication Date: 2016-03-16
江苏金海棠农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition, the commonly used propagation methods of Xifu crabapple are usually grafting and division propagation, and methods such as sowing, layering and root cuttings can also be used

Method used

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Embodiment Construction

[0026] The concrete steps of the present invention in practical operation are as follows:

[0027] The first thing to do is the preparatory work, select the leaves, petioles and stems of high-quality Xifu crabapple for use.

[0028] Prepare 5 types of media with MS as the basic medium:

[0029] Medium a: callus induction medium MS+BA3-5mg / L;

[0030] Medium b: cluster bud proliferation medium MS+BA1.0+NAA0.1mg / L;

[0031] Medium c: Cluster bud proliferation medium MS+BA0.5+NAA0.1mg / L;

[0032] Medium d: strong seedling medium 1 / 2MSmg / L;

[0033] Medium e: induction rooting medium 1 / 2MS+NAA0.2mg / L;

[0034] Among them, 3.0% sucrose and 0.8% agar need to be added to the above medium, and the pH value is 5.8; the culture temperature is between 22°C and 26°C, and the light time during the culture is 10h / d. The strength is 80Olx.

[0035] Acquisition of sterile materials: wash the leaves, petioles and stems of Begonia Xifu with appropriate amount of washing powder and water, ...

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Abstract

The present invention discloses a malus micromalus makino tissue culture method, which comprises: selecting malus micromalus makino with good growth as a material, taking the sterile blade cut block with a length of 0.5 cm and a width of 0.5 cm, the leaf stalk with a length of about 1 cm, and the stem section long strip, and uniformly inoculating into a culture medium a; after about 5 weeks, forming significant cluster buds, cutting the cluster buds into small blocks, respectively inoculating into culture mediums b and c, and proliferating; after four weeks, when the cut block enters a mass proliferation stage, carrying out transfer inoculation of the differentiated bud into a culture medium d, and culturing; after about 3 weeks, inoculating the root-free-like seedling with a height of about 3 cm and strong growth into a culture medium e; after 3 weeks, growing white fibrous roots on the base portion of the seedling; and placing the test-tube plantlet in a ventilation place for 3 days, taking out the small seedling, transplanting into a mixing matrix, and continuously culturing. With the method of the present invention, a large number of the high quality malus micromalus makino seedlings can be bred, and the survival rate is more than or equal to 95%.

Description

technical field [0001] The invention relates to the technical field of crabapple culture, in particular to a method for tissue culture of crabapple in Xifu. Background technique [0002] Xifu crabapple, also known as small fruit crabapple, is a plant of the genus Apple in the Rosaceae family. It was named after it grew in Xifu during the Jin Dynasty. Branches are erect, up to 2.5-5 meters. Branchlets are thin and cylindrical, pubescent when young, shedding when old, purplish red or dark brown, with sparse lenticels. Its flowers are in an umbel-shaped raceme, with 4-7 flowers gathered on the top of the branchlets, and the pedicels are 2-3 cm long. Its flowering period is from April to May, and the fruiting period is from August to September. [0003] Xifu crabapple stands tall among the crabapple flowers, like a slim girl. The flowers are red, the leaves are green, and the fruit is beautiful, regardless of whether it is planted alone, in a row or in a cluster, it is very...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王士岭
Owner 江苏金海棠农业科技有限公司
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