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Rice sheath blight effector gene rsia-np8 and its application

A technology of rice sheath blight bacteria and sheath blight, applied in the field of genetic engineering, can solve problems such as ineffective effects

Active Publication Date: 2021-04-13
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Strangely, monocot pathogens also carry NLPs genes, but their role in pathogenicity remains obscure because they do not cause cell necrosis

Method used

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  • Rice sheath blight effector gene rsia-np8 and its application
  • Rice sheath blight effector gene rsia-np8 and its application
  • Rice sheath blight effector gene rsia-np8 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Isolation and cloning of predicted effector genes

[0032] Under the ultra-clean workbench, pick a small amount of R. solani AG1IA cells with tweezers, put them into 50ml PDA medium, culture at 28°C and 200r / m for two days, filter them with four layers of gauze to collect the mycelium, and use Total RNA was extracted by grinding with liquid nitrogen. cDNA was obtained by reverse transcription using oligodT as a primer.

[0033] Primers are designed according to the predicted sequences, and the primer sequences are as follows:

[0034] Forward primer: 5'-ATGCGTTTTGCGACCGTAGC-3' (SEQ ID NO:3);

[0035] Reverse primer: 5'-GATGGCAACGCACGATTGTT-3' (SEQ ID NO: 4).

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Abstract

The invention provides a rice sheath blight effector gene RsIA‑NP8 and its application, the nucleotide sequence of the gene is shown in SEQ ID NO: 1; the amino acid sequence of the protein encoded by the gene is shown in SEQ ID NO: 2 shown. Through the cloning and functional analysis of the Rhizoctonia solani effector gene, the invention helps reveal the molecular mechanism of the specific interaction between rice sheath blight and rice varieties and its evolution.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a rice sheath blight effector gene RsIA-NP8 and its application. Background technique [0002] Rice sheath blight is a worldwide fungal disease of rice caused by Rhizoctonia solani Kühn, and is known as one of the three major diseases of rice. The disease has a wide distribution and occurs frequently in rice-growing countries in Asia, America and Africa in terms of global rice-growing regions. The disease was once the most important rice disease in the southern United States. The fungus can cause band leaf blight of corn, rice and sorghum, damp-off of cotton, aerial and stem rot of mung bean and soybean, sheath rot, cabbage heart rot, potato black spot and leaf rot Fusarium wilt. Sheath blight can infect a variety of crops and is related to its secretion of effector molecules. This molecule can modulate the host's innate immunity and enhance self-infection. It is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/31C07K14/37C12N15/82A01H5/00A01H6/46
CPCC07K14/37C12N15/8282
Inventor 郑爱萍魏苗苗刘尧王爱军马丽李平
Owner SICHUAN AGRI UNIV
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