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Application of dermal fibroblasts in the treatment of melanoma

A technology for fibroblasts and melanoma, applied in the field of application of dermal fibroblasts in the treatment of melanoma, can solve the problems of ESCC cell proliferation and invasion performance decline, etc.

Active Publication Date: 2022-01-11
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, some scholars have found that the proportion of ATF3 is down-regulated in esophageal squamous cell carcinoma (ESCC) lesions, and in vitro and in vivo studies, overexpression of ATF3 can lead to decreased proliferation and invasion of ESCC cells by regulating the expression of MDM2
ATF3 is also expressed in human dermis, but the inventors found that there is no report on its function in human dermal fibroblasts (HDFs) or melanoma-associated fibroblasts

Method used

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  • Application of dermal fibroblasts in the treatment of melanoma
  • Application of dermal fibroblasts in the treatment of melanoma
  • Application of dermal fibroblasts in the treatment of melanoma

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Experimental program
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Embodiment

[0069] 1. Materials and methods

[0070] 1.1 In vitro cell culture

[0071] Primary HDFs were isolated from foreskin tissue according to previously described protocols (Qian et al., 2019, Wen et al., 2018). HDFs and human melanoma cell lines Mel JuSo and UACC62 were cultured at 37°C and 5% CO2 with Dulbecco's modified Eagle's medium (DMEM, Gibco, USA), adding 10% FBS ( Bioindustry, Israel), penicillin (100 units / ml) and streptomycin (100 μg / ml) (ThermoFisher, USA) (full medium).

[0072] 1.2 In vivo tumor experiments

[0073] Tumor xenografts were grown and assayed as previously described (Wu et al., 2010). In order to test the inhibitory effect of increasing / decreasing IL-6 on the growth of melanoma cells in 8-week-old female nude mice (Charles River Laboratory, Wilmington, MA, USA) by HDFs overexpressing ATF3, 100 ng / kg / day / dose of mice, UACC62 melanoma cells and HDFs with different expression levels of ATF3 increasing / decreasing human IL-6 were mixed and injected into...

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Abstract

The invention provides an application of dermal fibroblasts in the treatment of melanoma, which belongs to the technical field of biomedicine. Through research, the present invention finds that in vitro, dermal fibroblasts expressing a high level of activating transcription factor 3 can inhibit the proliferation and migration of melanoma cells, accompanied by the downregulation of different cytokines (including IL‑6). In vivo, dermal fibroblasts with high expression of activator transcription factor 3 reduced tumor formation. Addition of recombinant IL‑6 to melanoma cells reversed those in vitro and in vivo effects, suggesting that activating transcription factor 3 expression in dermal fibroblasts regulates melanoma progression through the IL‑6 / STAT3 pathway, with promising practical applications value.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of dermal fibroblasts in the treatment of melanoma. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Originating from dysregulated proliferation of melanocytes, human cutaneous melanoma is one of the most aggressive and lethal tumors of the skin, accounting for more than 80% of skin cancer-related deaths. Despite recent advances in the treatment of melanoma, including the use of immune checkpoint inhibitors, treatment options for metastatic melanoma remain limited. Activating BRAF mutations are thought to be a major factor in the d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/10A61K35/33A61K45/06A61P35/00A61P35/04A61P43/00
CPCA61K35/33A61K45/06A61P35/00A61P35/04A61P43/00
Inventor 吴训伟俎廷建刘畅白福响冷雪弭军
Owner SHANDONG UNIV
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