A preparation method of gold/graphene quantum dots/mercaptopropionic acid/polyethyleneimine drug-loaded hydrogel that can be used for drug sustained release
A technology of graphene quantum dots and polyethyleneimine, applied in the fields of material synthesis and biomedicine, can solve problems such as side effects, and achieve the effect of simple and convenient preparation process
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Embodiment 1
[0022] The preparation of gold / graphene quantum dots / mercaptopropionic acid / polyethyleneimine drug-loaded hydrogel includes the following steps:
[0023] (1) Dissolve 2g of citric acid monohydrate in 15mL of ultrapure water, then move the clear solution into a crucible, take it out in a muffle furnace at 175°C for 2h heating time, cool to room temperature and dissolve in ultrapure water to configure A graphene quantum dot solution with a concentration of 20 mg / mL was prepared for later use.
[0024] (2) 2 mL of 20 mg / mL chloroauric acid solution and 2 mL of the graphene quantum dot solution prepared in step (1) were evenly mixed, irradiated with ultraviolet light for 5 min, centrifuged to remove the supernatant to obtain gold / graphene quantum dots, and A gold / graphene quantum dot solution with a concentration of 1.5 mg / mL is configured for use; for example, the obtained gold / graphene quantum dots are washed with ultrapure water for several times and centrifuged to remove the s...
Embodiment 2
[0028] The temperature-time response graphs of NIR light-irradiated graphene quantum dots, gold / graphene quantum dots, and gold / graphene quantum dots / mercaptopropionic acid / polyethyleneimine drug-loaded hydrogels include the following steps:
[0029] The preparation process of graphene quantum dots, gold / graphene quantum dots, and gold / graphene quantum dots / mercaptopropionic acid / polyethyleneimine drug-loaded hydrogel is the same as that of Example 1.
[0030]Graphene quantum dots, gold / graphene quantum dots, and gold / graphene quantum dots / mercaptopropionic acid / polyethyleneimine drug-loaded hydrogels were prepared into solutions with a concentration of 1.5 mg / mL, and 5 mL of the solutions were taken out and placed separately. In a beaker, at room temperature of 25°C, the side of the thermometer for temperature measurement is completely immersed in the solution, irradiated with a near-infrared laser with a wavelength of 808 nm and a power of 1W at a distance of 2 cm from the be...
Embodiment 3
[0033] The in vitro drug release of gold / graphene quantum dots / mercaptopropionic acid / polyethyleneimine drug-loaded hydrogels at different pH values at 37°C includes the following steps:
[0034] The preparation process of gold / graphene quantum dots / mercaptopropionic acid / polyethyleneimine drug-loaded hydrogel is the same as that of Example 1.
[0035] (1) Weigh 20mg of gold / graphene quantum dots / mercaptopropionic acid / polyethyleneimine drug-loaded hydrogel respectively, place them in a dialysis bag, and place the dialysis bag in 30mL of phosphate buffer solutions with different pH, respectively, The drug was released in vitro by magnetic stirring at a temperature of 37°C. The pH of the phosphate buffer solution was 5.0, 6.2 and 7.4, respectively, and the drug was released for 12 hours.
[0036] (2) Take a sample every 1 hour, take out 3 mL of solution for each sampling, measure the amount of cytarabine released, and supplement 3 mL of fresh phosphate buffer solution at the ...
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