Luminous bacterium freeze-dried powder kit and preparation method thereof

Abstract

The invention relates to the technical field of water quality detection kits, and particularly relates to a luminous bacterium freeze-dried powder kit and a preparation method thereof. The preparationmethod comprises the following steps of recovering strain freeze-dried powder, performing inoculated culture to obtain luminous bacterium liquid, performing 256-fold diluting on the luminous bacterium liquid, and detecting the luminous quantity of the diluted luminous bacterium liquid; sucking a certain volume of the diluted luminous bacterium liquid, and performing refrigerated centrifugation toobtain thalli; mixing the thalli with a protective agent to obtain a thallus mixed solution; performing vacuum freeze drying on the thallus mixed solution to obtain luminous bacterium freeze-dried powder; and forming the luminous bacterium freeze-dried powder kit by the luminous bacterium freeze-dried powder, a diluent, an osmotic pressure regulating solution and a quality control standard substance solution. According to the invention, the stability regulation and control of important parameters such as the initial luminous quantity, the CF value, the positive control value and the negativecontrol value of the kit in the production process are realized, the performance consistency between different preparation batches can be ensured, and the situation that deviation occurs when different batches of kits are used for evaluating the toxicity of a same water body is effectively avoided.

Description

technical field [0001] The invention relates to the technical field of water quality detection kits, in particular to a luminescent bacteria freeze-dried powder kit and a preparation method thereof. Background technique [0002] With the increasingly serious water pollution, a large amount of toxic and harmful pollutants are discharged into lakes, rivers and oceans, causing harm to the ecological balance and organisms of aquatic ecosystems. Compared with traditional detection methods for fish, fleas and plankton, The luminescent bacteria method for detecting water quality biological toxicity has the advantages of fast and simple detection, and has been widely used at home and abroad. [0003] The luminescent bacteria method uses a sensitive photoelectric measurement system to measure the influence of poisons on the luminous intensity of luminescent bacteria, and judge the toxicity of poisons. At present, in the experiment of determining the acute toxicity of water quality b...

AI Technical Summary

Problems solved by technology

However, the preparation method of the kit only includes the preparation of the freeze-dried powder of the kit, and does not mention the technical content of the important parameters of the kit during the production process, resulting in poor repeatability between batches of the kit.

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