A luminescent bacteria freeze-dried powder kit and preparation method thereof

Abstract

The invention relates to the technical field of water quality detection kits, in particular to a luminescent bacteria freeze-dried powder kit and a preparation method thereof. The preparation method comprises: resuscitating the freeze-dried powder of the strain, inoculating and culturing it to obtain the luminescent bacteria liquid, and detecting the luminescence amount after 256-fold dilution; drawing a certain volume of the luminescent bacteria liquid and performing refrigerated centrifugation to obtain the bacterium; Mix the cells with the protective agent to obtain a bacterial cell mixture; vacuum freeze-dry the bacterial cell mixture to obtain a luminescent bacteria freeze-dried powder; the obtained luminescent bacteria freeze-dried powder, diluent, osmotic pressure adjustment solution, and quality control standard substance solution Luminescent Bacteria Lyophilized Powder Kit. The present invention realizes the stable control of important parameters such as the initial luminescence amount, CF value, positive control value and negative control value of the kit during the production process, can ensure the performance consistency between different preparation batches, and effectively avoids the use of different batches There will be deviations when using different kits to evaluate the toxicity of the same water body.

Description

technical field [0001] The invention relates to the technical field of water quality detection kits, in particular to a luminescent bacteria freeze-dried powder kit and a preparation method thereof. Background technique [0002] With the increasingly serious water pollution, a large number of toxic and harmful pollutants are discharged into lakes, rivers and oceans, causing harm to the ecological balance and organisms of aquatic ecosystems. Compared with traditional detection methods for fish, fleas and plankton, The luminescent bacteria method for the detection of water quality biological toxicity has the advantages of rapid and simple detection, and has been widely used at home and abroad. [0003] The luminescent bacteria method uses a sensitive photoelectric measuring system to measure the influence of the poison on the luminescent intensity of the luminescent bacteria, and judge the toxicity of the poison. At present, in the experiment of the luminescent bacteria metho...

AI Technical Summary

Problems solved by technology

However, the preparation method of the kit only includes the preparation of the freeze-dried powder of the kit, and does not mention the technical content of the important parameters of the kit during the production process, resulting in poor repeatability between batches of the kit.

Images