Treatment method and treatment agent for biological specimen

A technology for biological specimens and treatment methods, applied in the field of biological specimen treatment methods and treatment agents, can solve the problems of horn plug specimen structure damage, microbial loss, difficult research, etc., to reduce trauma, avoid ethical issues, and reduce pain Effect

Active Publication Date: 2020-06-05
SHANGHAI DERMATOLOGY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In the process of studying the structure and microorganisms of acne horn plugs, the researchers used the above-mentioned traditional paraffin embedding and sectioning methods to make specimens, but encountered the structural damage of the horn plug specimens and the problem of microbe loss, the structure and microbe integrity of the acne keratin plug cannot be maintained, and it is difficult to carry out follow-up research

Method used

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  • Treatment method and treatment agent for biological specimen
  • Treatment method and treatment agent for biological specimen
  • Treatment method and treatment agent for biological specimen

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Experimental program
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Effect test

Embodiment 1

[0037] The horn plug of acne is a characteristic structure of acne vulgaris, which contains a lot of microbial information, which is of great significance for exploring the etiology and mechanism of acne. The traditional sampling method involves histology, which requires the use of a trephine drill to punch a hole in the skin for a trephine biopsy sample, which is very difficult to accept on the face, which will damage the patient's appearance, cause skin damage and pain, so there is a Ethical issues; even if biopsies can be taken with patient consent, large sample sizes are not possible, hampering studies with large samples.

[0038] If the acne horn plug is separated by non-invasive extrusion method, and there is no suitable coating method for treatment, the integrity of its structure and contained microorganisms cannot be guaranteed by sectioning. Because the horn plug structure is loose and lacks connexin, in the process of traditional embedding method, due to the use of a...

Embodiment 2

[0056] The embedding technology provided by the present invention can not only be used in the preparation of acne horn plug specimens, but also can be widely used in the preparation of other tiny biological tissue specimens. Use smaller specimens, obtain more information, and bring convenience to clinical research and diagnosis.

[0057] In this example, a single pilosebaceous gland with a diameter of only a few hundred microns and a single pilosebaceous gland was successfully coated and sliced ​​using a double coating method similar to that in Example 1. The obtained results are as follows: figure 2 shown. figure 2 The structure and shape of the pilosebaceous glands in the image are very complete, which can reach the image quality obtained by traditional slicing techniques. This work would not have been possible without the use of this method.

[0058] The present invention is used for the coating and processing of extremely small and precious skin and whole body tissue s...

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Abstract

The invention provides a treatment method and a treatment agent for a biological specimen. According to the treatment method provided by the invention, before a biological specimen is in contact witha cross-linking agent, the surface of the biological specimen is coated with gelatin to form a gelatin coating layer. The treatment method comprises the following main steps: 1, wrapping a biologicalspecimen with heated gelatin hydrogel; and 2, cooling and solidifying the gelatin hydrogel, and then placing the biological specimen coated with the gelatin in a cross-linking agent for cross-linkingtreatment so as to enable the gelatin coating layer to form a fixed insoluble cross-linked product. The biological specimen treating agent comprises gelatin, and the concentration of the gelatin ranges from 15% to 40%. The treatment method and the treatment agent for the biological specimen have the following beneficial effects: (1) pain and trauma of a patient are reduced; (2) avoiding of ethicalproblems; and (3) large-scale research becomes possible.

Description

technical field [0001] The invention relates to a treatment method and agent for biological specimens, in particular to using gelatin to coat tiny or extremely thin biological specimens to maintain the tissue structure or internal components of the specimens and the integrity of microorganisms, and belongs to the field of biotechnology. Background technique [0002] When using a microscope to observe, specimen sections are required. When making specimen slices or ultra-thin sections, it is difficult to make slices with uniform thickness because the tissue is soft, or the local softness and hardness are uneven. Therefore, it is necessary to soak the inside of the tissue with a certain substance, so that the whole tissue can be uniformly hardened, so that it can be cut into thin slices with a sharp knife. This soaking substance is called an embedding agent. [0003] The paraffin slice method is a method in which the material is fixed, dehydrated, soaked in wax, and then embedd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/36G01N23/2202
CPCG01N1/2806G01N1/286G01N1/30G01N1/36G01N23/2202G01N2001/2873
Inventor 许德田王秀丽
Owner SHANGHAI DERMATOLOGY HOSPITAL
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