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Bacteroides vulgaris strain and its application in preparation of inflammatory bowel disease therapeutic drug

A technology of Bacteroides vulgaris and inflammatory bowel disease, applied in the field of microbiology, can solve the problem of uneven effects, achieve the effect of reducing activity index, reducing the degree of inflammatory damage, and excellent application prospects

Active Publication Date: 2021-08-27
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of fecal transplantation in the treatment of IBD has been reported, but the effect is variable

Method used

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  • Bacteroides vulgaris strain and its application in preparation of inflammatory bowel disease therapeutic drug
  • Bacteroides vulgaris strain and its application in preparation of inflammatory bowel disease therapeutic drug
  • Bacteroides vulgaris strain and its application in preparation of inflammatory bowel disease therapeutic drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1. Isolation, screening and identification of Bacteroides vulgaris

[0019] 1. Isolation of Bacteroides vulgaris

[0020] 1) Prepare brain-heart infusion agar medium, after 121°C, 15min autoclaving, cool to 50°C, first add 5ug / ml vancomycin, shake gently, then add 5% defibrinated sheep blood, mix Pour into the petri dish after mixing;

[0021] 2) Take the 10 diluted by gradient -4 56ul of the feces sample was coated on the culture medium prepared in step 1);

[0022] 3) At 37°C, 0.5% CO 2 Cultivate in the environment for 48h;

[0023] 4) Pick a single colony that is moist and round and raised for transfer.

[0024] 2. Strain preservation

[0025] In this laboratory, the culture medium containing 25% glycerol is used as the bacteria preservation solution to carry out the cryopreservation of the strains, and the method is as follows:

[0026] 1) Sterilize the 2mL sterile tube by autoclaving at 121°C for 15 minutes for use;

[0027] 2) After the bacteria ar...

Embodiment 2

[0052] Example 2, Bacteroides vulgaris can effectively improve DSS-induced colitis in mice

[0053] 1. Research methods:

[0054] Thirty-eight 6-8 week old female SPF C57BL / 6J mice were randomly divided into two groups, PBS group (n=20) and Bacteroids group (n=18) after 5 days of adaptation. All mice were given drinking water containing 3% DSS solution every day from the 0th day, and the DSS solution was changed once every 3 days for 6 consecutive days. Each rat was given 200 μL of heat-inactivated bacterial solution containing 108 CFU per day.

[0055] The mouse colon tissues were taken and fixed in 10% formalin solution. Send to Beijing Boao Saien Technology Co., Ltd. for making pathological slices. The process is as follows: the colonic tissue is made into a wax block after being dehydrated, transparent, soaked in wax. Cut into 4 μm slices with a microtome. Hematoxylin-eosin staining was then performed. HE sections were evaluated blindly by two experienced pathologist...

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PUM

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Abstract

The invention discloses a Bacteroides probiotic strain, the preservation number of which is CGMCC NO.17140, the preservation date is January 07, 2019, the preservation classification is named Bacteroides vulgaris, and the preservation unit is China Microbial Culture Collection Management Committee Center for General Microbiology. The bacterial strain is harmless to animals, and has the remarkable effect of improving and regulating intestinal inflammation. The invention also discloses the application of the bacterial strain in the preparation of medicines and health care products for preventing and treating inflammatory bowel disease.

Description

technical field [0001] The invention relates to a potential probiotic and its application, belonging to the field of microorganisms. Background technique [0002] Inflammatory bowel disease (Inflammatory Bowel Disease, IBD) is a non-specific intestinal inflammatory disease of unknown etiology, including Crohn's disease (Crohn's Disease, CD) and ulcerative colitis (Ulcerative Colitis, UC). There is no ideal treatment, requiring long-term treatment and frequent hospitalization. Commonly used drugs such as aminosalicylic acid and corticosteroids are used for mild to moderate verification, and anti-inflammatory drugs such as TNF inhibitors and immunosuppressant drugs are used for those who are ineffective, but long-term use may cause allergic reactions or liver damage side effects. New drugs capable of protecting enteritis have become a technical demand in this field. [0003] The pathogenesis of IBD is complex, and it is generally believed to be related to multiple factors su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/741A61P29/00A61P1/00A61P1/04C12R1/01
CPCA61K35/741A61P1/00A61P1/04A61P29/00C12N1/20C12N1/205C12R2001/01
Inventor 任志鸿徐建国李先平杨晶张桂卢珊
Owner ICDC CHINA CDC
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