Application of lactobacillus acidophilus in preparation of food or medicine for preventing or treating antibiotic-associated diarrhea
A technology of Lactobacillus acidophilus and antibiotics, applied in the field of microorganisms, can solve limited problems, achieve the effect of alleviating antibiotic-associated diarrhea, reducing disease activity index, and improving the lesions of antibiotic-associated diarrhea
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Embodiment 1
[0048] The present embodiment provides a Lactobacillus acidophilus LA85 freeze-dried powder, and its preparation method is as follows:
[0049] The Lactobacillus acidophilus LA85 strain was inoculated into the medium according to the inoculum amount of 3% of the total mass of the MRS medium, and cultured at 37 ° C for 18 hours to obtain a culture solution; the culture solution was centrifuged to obtain the bacteria; the bacteria were used seaweed Sugar freeze-drying protective agent (containing trehalose 100g / L, skimmed milk powder 100g / L, solvent is water) is resuspended (the mass ratio of freeze-drying protective agent and thalline is 2:1) to obtain resuspended liquid; The liquid was freeze-dried by vacuum freezing method to obtain Lactobacillus acidophilus LA85 freeze-dried powder, and the number of viable bacteria was detected as 300 billion CFU / g.
Embodiment 2
[0072] The present embodiment provides a solid beverage with the efficacy of relieving antibiotic-related diarrhea, and its preparation method is as follows:
[0073] Mix 0.1 g of Lactobacillus acidophilus freeze-dried powder prepared in Example 1 with 1.9 g of inulin to homogenize, and the number of viable bacteria detected is 1 billion CFU / g.
Embodiment 3
[0075] The present embodiment provides a solid beverage with the efficacy of relieving antibiotic-related diarrhea, and its preparation method is as follows:
[0076] Mix 0.1 g of Lactobacillus acidophilus freeze-dried powder prepared in Example 1 with 1.9 g of resistant dextrin to homogenize, and the number of viable bacteria detected is 1 billion CFU / g.
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