Phosphorus-solubilizing, growth-promoting and high-adaptability corn rhizosphere growth-promoting bacterium and application thereof
A technology for dissolving phosphorus and microbial strains, applied in the field of microorganisms, can solve problems such as low phosphorus utilization efficiency
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Embodiment 1
[0067] Example 1, screening of phosphorus-dissolving and growth-promoting bacterial strains and identification of other functions
[0068] Northeast my country is one of the main corn planting areas in China, and the black soil in Northeast China urgently needs to take effective protection measures to curb the degradation of black soil. Aiming at corn production and soil status in Northeast China, the invention screens out multifunctional corn rhizosphere growth-promoting phosphorus-dissolving bacteria by isolating phosphorus-dissolving bacteria from corn rhizosphere soil and measuring the strain's functions such as IAA production and ecological adaptability.
[0069] 1. Materials and methods
[0070] 1. Experimental materials
[0071] (1) Collection of soil samples
[0072] On September 15, 2017, in Sankeshu Village, Lishu County, Siping City, Jilin Province, the corn rhizosphere soil was collected by the 5-point sampling method, the samples were mixed, put into sterile zip...
Embodiment 2
[0132] Embodiment 2, identification and preservation of bacterial strain MGPR2
[0133] 1. Identification method of strain MGPR2
[0134] 1. Identification of morphological characteristics
[0135] The bacterial strain MGPR2 screened in Example 1 was inoculated on LB solid medium, cultured at 37° C. for 3 days, and the colony morphology was observed.
[0136] 2. Identification of physiological and biochemical characteristics
[0137] The bacterial strain MGPR2 that screening obtains in embodiment 1 is carried out Gram stain, and utilizes Biolog technology to carry out 94 kinds of phenotypic tests to this bacterial strain, the Gen III microwell plate that adopts comprises: 71 kinds of carbon source utilization tests (table 5, 1 -9 columns) and 23 chemical sensitivity tests (Table 5, columns 10-12). All necessary nutrients and biochemical reagents are pre-filled and dried in 96 wells. The tetrazole-based redox dyes in the pores indicate the degree of utilization of carbon so...
Embodiment 3
[0151] Preparation and application of embodiment 3, MGPR2 bacterial agent
[0152] 1. Preparation of MGPR2 bacterial agent
[0153] Inoculate the Enterobacter sp. MGPR2 strain into LB liquid medium supplemented with tryptophan at a final concentration of 0.1 mg / mL and culture it on a shaking table (37°C, 180r / min) for 24-48 hours to obtain MGPR2 Liquid bacteria. The concentration of Enterobacter sp. MGPR2 in the liquid bacterial agent is 1×10 10 -1×10 11 cfu / mL.
[0154] 2. Field application of MGPR2 bacterial agent
[0155] 1. Experimental materials and methods
[0156] Experimental material: Maize variety Zhengdan 958.
[0157] Experimental location: Sankeshu Village, Lishu County, Siping City, Jilin Province.
[0158] Experimental method: On April 30, 2018, seeds were sown in the experimental field according to conventional methods.
[0159] The experimental field is divided into two parts, phosphorus deficiency area and normal fertilization area.
[0160] Phosphorus...
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