A rapid determination method for temperature-resistant trait index abt of Chlamys farreri

A method for rapid measurement of scallops and scallops, which is applied in the measurement of pulse rate/heart rate, diagnostic recording/measurement, medical science, etc. It can solve the problems of lack of temperature-resistant ABT and other problems, and achieve the effect of improving the measurement method and improving the measurement efficiency.

Active Publication Date: 2021-08-27
OCEAN UNIV OF CHINA
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Problems solved by technology

Solve the problem of the lack of standardized high-throughput measurement of temperature-resistant traits ABT in the current marine economic shellfish farming process, so that ABT can be used as a trait index, just like body size traits such as shell height, shell length, shell width, and weight. Genome-wide selective breeding of scallop

Method used

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  • A rapid determination method for temperature-resistant trait index abt of Chlamys farreri
  • A rapid determination method for temperature-resistant trait index abt of Chlamys farreri
  • A rapid determination method for temperature-resistant trait index abt of Chlamys farreri

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Embodiment 1

[0039] 1. The connection of the scallop heartbeat detection instrument:

[0040] (1) The interface end of the infrared sensor Infrared Sensors (CNY-70) is connected to the Heartbeat monitor amplifier (AMP-03) through RJ11 connection;

[0041] (2) Both ends of the Heartbeat monitor amplifier (AMP-03) and PowerLab 16035 sixteen-channel research-type high-speed recording host (including LabChart Pro professional version) are connected to each other by BNC interface;

[0042] (3) PowerLab 16035 16-channel research high-speed recording host (including LabChart Pro Professional Edition) is connected to the computer via USB connection (LabChart v8 for Windows is installed).

[0043] 2. Standardization of scallop heartbeat detection method:

[0044] (1) Remove the attached organisms on the shell of the experimental scallops (N ≥ 18, similar in size, and have different genetic backgrounds), and put them under experimental conditions for 5-7 days to adapt to the experimental environmen...

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Abstract

The present invention establishes a new method for rapid determination of the temperature-resistant trait index ABT of Chlamys farreri. The method of the present invention utilizes a heart rate HR of 11°C 11℃ To calculate ABT, the calculation formula is as follows: ABT=‑0.427*HR 11℃ +33.159(R 2 = 0.5028). The invention carries out standardized, low-cost and high-throughput measurement on the temperature-resistant trait ABT of Chlamys farreri, and uses the obtained estimated value of ABT as the basis for selection of parent shellfish in the breeding process. The present invention improves the equipment and the measuring method of the existing scallop ABT measuring system. The rapid measuring method of the temperature-resistant trait index ABT of Chlamys farreri established in the present invention has a measuring rate of about 1 min / individual, which significantly improves the temperature-resistant traits of scallop. Assay efficiency of ABT. For the first time, a fast estimation method for ABT based on scallop heart rate index was proposed. This method can provide a research basis for the breeding of stress-resistant varieties of Chlamys farreri and the establishment of resistance index system.

Description

Technical field: [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a rapid determination method for the temperature-resistant trait index ABT of Chlamys farreri. Background technique: [0002] The shellfish industry is an important part of my country's mariculture industry, and the aquaculture production accounts for about 71.09% of the total mariculture production (China Fishery Statistical Yearbook, 2019). Shellfish farming represented by scallops once led the third wave of marine aquaculture in my country and promoted the rapid development of the aquaculture industry. Chlamys farreri is the earliest scallop species cultivated in China, and it belongs to the leading shellfish culture species in the northern coast of my country. Since the end of the 20th century, Chlamys farreri often died on a large scale during the high temperature season in summer, which not only brought huge economic losses to farmers, but also restri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/54A61B5/318A61B5/0205
CPCA61B5/0205A61B5/024A61B2503/40A61B2503/42A01K61/54A61B5/318Y02A40/81
Inventor 邢强朱星海彭程陆维黄晓婷张玲玲包振民
Owner OCEAN UNIV OF CHINA
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