Culture medium for culturing Akkermansia muciniphila, and application method and applications of culture medium
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A technology of medium and mucin, applied in the field of medium for cultivating Akkermansia muciniphila
Active Publication Date: 2020-06-19
SUZHOU PRECISION BIOTECH CO LTD
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However, Akkermansia muciniphila can still be cultured by using the above-mentioned medium without adding bovine brain heart infusion agar dry powder and porcine gastric mucin, but the OD of Akk bacteria can be obtained 600 value is still low
[0006] Therefore, there ar
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Embodiment 1
[0062] The present embodiment provides a culture medium, the culture medium comprising:
[0063] 7.4g brain heart infusion, 0.8g porcine gastric mucin, 0.3g L-cysteine hydrochloride, 1.0g threonine, 0.015% vitamin K 1 , 1.0g N-acetyl-D-glucosamine, add water to make up to 200mL, stir for 30min and mix well, transfer to a conical flask, and sterilize.
Embodiment 2
[0065] The present embodiment provides a culture medium, the culture medium comprising:
[0066] 7.4g brain heart infusion, 0.8g porcine gastric mucin, 0.3g L-cysteine hydrochloride, 1.0g threonine, 0.015% vitamin K 1 , 1.0g N-acetyl-D-glucosamine, 0.1mL trace elements, add water to make up to 200mL, stir for 30min and mix well, transfer to a conical flask, and sterilize.
Embodiment 3
[0068] The present embodiment provides a culture medium, the culture medium comprising:
[0069] 7.4g brain heart infusion, 0.8g porcine gastric mucin, 0.3g L-cysteine hydrochloride, 1.0g threonine, 0.015% vitamin K 1 , 1.0g N-acetyl-D-glucosamine, 0.1mL trace element, Tween-80 with a mass fraction of 0.015%, add water to make up to 200mL, stir for 30min and mix well, transfer to a conical flask, and sterilize.
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Abstract
The invention provides a culture medium for culturing Akkermansia muciniphila, and an application method and applications of the culture medium. The culture medium includes brain heart infusion, porcine gastric mucin, L-cysteine hydrochloride, threonine, N-acetyl-D-glucosamine and vitamins. Through the adding of the L-cysteine hydrochloride, the threonine, the N-acetyl-D-glucosamine and the vitamins in the culture medium, the Akkermansia muciniphila can still obtain enough nutrients in the culture medium with low concentrations of the brain heart infusion and the porcine gastric mucin, so thatthe normal growing and reproduction of strains can be guaranteed. After the Akkermansia muciniphila is cultured by the provided culture medium, the OD value of the obtained bacterial solution is 0.8211-0.8741.
Description
technical field [0001] The invention belongs to the technical field of microbial culture medium, and in particular relates to a culture medium for Akkermansia muciniphila as well as a use method and application thereof. Background technique [0002] Akkermansia muciniphila, referred to as Akk bacteria, is a Gram-negative strict anaerobic coccus. In 2004, Professor Derian and her team isolated Akk bacteria from the human gut, which are ubiquitous in the human digestive tract, accounting for about 3-5%, and can degrade mucin in the human gut, which is closely related to obesity, diabetes, inflammation and Metabolic disorders are negatively correlated, and have the effects of delaying aging, inhibiting neurodegenerative diseases, and reducing lipids and weight loss. [0003] Low levels of Akk bacteria in the gut may lead to thinning of the mucosal layer, leading to a weakened gut barrier function, making it easier for toxins in the gut to enter the body. Therefore, Akk bacter...
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