A device for establishing an animal model of silicosis and its evaluation method

The technology of an animal model and a generating device is applied in the device field of silicosis research, which can solve the problems of complex pathogenesis of silicosis, and achieve the effects of simple and reasonable structure, easy preparation and operation, and low cost.

Active Publication Date: 2022-05-27
山东省职业卫生与职业病防治研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The pathogenesis of silicosis is very complex and has not yet been fully elucidated

Method used

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  • A device for establishing an animal model of silicosis and its evaluation method
  • A device for establishing an animal model of silicosis and its evaluation method
  • A device for establishing an animal model of silicosis and its evaluation method

Examples

Experimental program
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Effect test

Embodiment 1

[0041] A device for establishing an animal model of silicosis is mainly composed of three parts, the upper part is a silica dust aerosol generating device 1, the middle part is an animal dusting cabinet 2, and the lower part is a waste gas treatment device 3.

[0042] The silica dust aerosol generating device 1 is a silica dust generating device, which consists of a silica dust stirring device 5 with a stainless steel scraper 7, a stainless steel leakage plate 6 with holes at the bottom and a cylindrical tank 4, and the test substance is free silica It is composed of quartz sand with a content of more than 99%, the particle size is 8000 mesh, and the dust concentration is 1000 mg / m2 3 , according to the concentration of the sampling port, adjust the rotation speed of the scraper 7 to obtain the silica dust aerosol with the concentration level; the hole on the stainless steel plate is 2mm in diameter, and the position is on the side of the scraper 7; the circular tank 4 is made ...

Embodiment 2

[0046] Through the establishment device for silicosis animal model, the method for establishing and evaluating an animal model of silicosis includes two parts: establishment and evaluation of an animal model in the inflammatory stage of silicosis, and establishment and evaluation of an animal model in the fibrosis stage of silicosis; The experimental animals were put into animal fixed bottles, and the free silica content was more than 99% and the concentration was 1000mg / m 3 Silica dust is contaminated by natural inhalation.

[0047] The evaluation indicators for model establishment are as follows.

[0048] 1. Selection of evaluation indicators.

[0049] Hydroxyproline (HYP) content in lung tissue: The HYP content in lung tissue was detected on the 3rd, 14th, 28th and 60th days. With the extension of dust exposure time, the content showed an upward trend, and the highest content was at 60 days.

[0050] Transforming growth factor β1 (TGF-β1) content in lung tissue: The TGF-β...

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Abstract

The invention discloses a device for establishing an animal model of silicosis and an evaluation method thereof. The device is divided into three connected parts from top to bottom. Exhaust gas treatment device; silica dust aerosol generating device is used for silica dust generation, including the tank body and the silica dust stirring device installed in the tank body, and the drain plate installed at the bottom of the tank body; there are upper and lower layers inside the dust dyeing cabinet, The upper layer is a silica dust aerosol mixing device, and the lower layer is a fixed dust collection device for rats; the exhaust gas treatment device has an opening to connect to the filter dust bag; all connections are sealed. The invention also provides a method for establishing and evaluating silicosis animal models through the device. The invention can conveniently and simply establish an effective silicosis animal model, provides a good working basis for the research on the pathogenesis and treatment of silicosis, and provides an objective, direct and reliable standard for the silicosis animal model.

Description

technical field [0001] The present invention relates to a device and method for silicosis research, in particular, to a device for establishing an animal model of silicosis and an evaluation method thereof. Background technique [0002] Silicosis is an occupational disease caused by inhalation of quartz dust in the lungs, and there is inflammation and scarring in the form of pulmonary nodular lesions in the upper lobes. a type of disease. The lungs are one of the main internal organs of the human body. Inhaled air undergoes gas exchange in the alveoli, expelling carbon dioxide, oxygenating the blood, and sending it back to the heart. With the beating of the heart, oxygenated blood is transported to all parts of the body. People suffering from pneumoconiosis have insufficient oxygen in the blood and excessive carbon dioxide due to the obstruction of gas exchange. If this phenomenon persists for a long time, it will lead to right ventricular hypertrophy or pulmonary heart o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K1/03B01D46/02G01N1/30G01N33/68
CPCA01K1/03B01D46/02G01N1/30G01N33/68G01N33/6872G01N2333/495
Inventor 张海东杨治峰张津玮
Owner 山东省职业卫生与职业病防治研究院
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