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Method for simultaneously detecting contents of effective components in angelica sinensis

A technology of active ingredients and content, applied in the field of simultaneous detection of active ingredient content in Angelica sinensis, can solve the problems of unfavorable quality control of Angelica sinensis medicinal materials, single detection index of Angelica sinensis medicinal materials, etc.

Pending Publication Date: 2020-07-10
云南昊辰制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to comprehensively control the quality of angelica medicinal materials, the technical problem to be solved by the present invention is that the detection index of angelica medicinal materials in the prior art is single, which is not conducive to the quality control of angelica medicinal materials, and then provide a fast, comprehensive and targeted detection method

Method used

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  • Method for simultaneously detecting contents of effective components in angelica sinensis
  • Method for simultaneously detecting contents of effective components in angelica sinensis
  • Method for simultaneously detecting contents of effective components in angelica sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The method for simultaneously detecting the content of active ingredients in Angelica sinensis in this embodiment is to measure tryptophan, chlorogenic acid, ferulic acid, ligusticolide I, lisanthenolide H, and emeraldrone in Yunnan Weixi angelica medicinal materials by HPLC. Hu Su, Z-ligustilide, butenylphthalide content. Specifically include the following steps:

[0063] Step (1) Preparation of reference substance solution:

[0064] Accurately weigh the appropriate amount of tryptophan, chlorogenic acid, ferulic acid, ligustilide I, listonin H, imperatorin, Z-ligustilide, butenylphthalide reference substance, add 70 %Methanol is formulated to contain 50 μg of tryptophan, 70 μg of chlorogenic acid, 55 μg of ferulic acid, 16 μg of ligustilide I, 5 μg of ligustilide H, 190 μg of imperatorin, 500 μg of Z-ligustilide, and 500 μg of butene per 1 mL. A solution of 50 μg of phthalide was obtained.

[0065] Step (2) preparation of need testing solution:

[0066] Take test ...

Embodiment 2

[0077] The method for simultaneously detecting the content of active ingredients in Angelica sinensis in this embodiment is to measure tryptophan, chlorogenic acid, ferulic acid, ligusticolide I, lisanthemum listonin H, and prunus chinensis in the medicinal materials of Angelica sinensis by HPLC. Sustained element, Z-ligustilide, butenylphthalide content. Specifically include the following steps:

[0078] Step (1) Preparation of reference substance solution:

[0079] Accurately weigh an appropriate amount of tryptophan, chlorogenic acid, ferulic acid, ligustilide I, listonin H, imperatorin, Z-ligustilide, butenylphthalide reference substance, add 100 %Methanol was formulated to contain 4.22 μg of tryptophan, 6.02 μg of chlorogenic acid, 4.60 μg of ferulic acid, 1.27 μg of ligustin I, 0.44 μg of listonin H, 16.8 μg of imperatorin, and 16.8 μg of imperatorin per 1 mL. A solution of 44.0 μg of ligustilide and 4.11 μg of butenylphthalide was obtained. Step (2) preparation of ne...

Embodiment 3

[0091] The method for simultaneously detecting the content of active ingredients in Angelica sinensis in this embodiment is to measure tryptophan, chlorogenic acid, ferulic acid, chuanxiong lactone I, and chuanxiong in Gansu, Yunnan Lijiang, Ruili, and Dali angelica medicinal materials by HPLC. Ester H, imperatorin, Z-ligustilide, butenylphthalide content. Including the following steps:

[0092] Step (1) Preparation of reference substance solution:

[0093] Accurately weigh an appropriate amount of tryptophan, chlorogenic acid, ferulic acid, ligustilide I, ligustilide H, imperatorin, Z-ligustilide, butenylphthalide reference substance, add 50 %Methanol was formulated to contain 84.40 μg of tryptophan, 120.40 μg of chlorogenic acid, 92.00 μg of ferulic acid, 25.30 μg of chuanxionglide I, 8.72 μg of chuanxionglide H, 321.60 μg of imperatorin, and 321.60 μg of imperatorin per 1 mL of methanol. A solution of 880 μg of ligustilide and 82.26 μg of butenylphthalide was obtained.

...

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Abstract

The invention relates to a method for simultaneously detecting the contents of effective components in angelica sinensis. The effective components comprise organic acids, phthalides, amino acids and coumarins. The method comprises the following steps: preparing a test solution, preparing a reference solution and detecting a sample. According to the method established by the invention, the index components of organic acids, phthalides, amino acids and coumarins in the angelica sinensis are simultaneously detected for the first time, so that the quality of the medicinal material of angelica sinensis is more comprehensively evaluated and controlled. The detection indexes selected by the invention are components with high content and pharmacological activity in the angelica sinensis. A basis can be provided for safety and effectiveness of clinical application of the angelica sinensis medicinal material. The detection time and cost are saved, and the method is accurate, simple and feasible.The method has the advantages of high stability, repeatability recovery rate and specificity.

Description

technical field [0001] The invention relates to a method for simultaneously detecting the content of active ingredients, in particular to a method for simultaneously detecting the content of active ingredients in angelica, belonging to the field of quality inspection of traditional Chinese medicinal materials. Background technique [0002] Angelica sinensis is the dried root of Angelica sinensis (Oliv.) Diels, an umbrella plant. It is mainly produced in Gansu, and it is also planted in Yunnan, Sichuan, Hubei and other provinces. In addition to Gansu angelica, due to its excellent quality, cloud angelica has gradually become an important part of the angelica market, and the planting area is constantly expanding. In addition to "Mingui", most of the products sold in the market are "Yundanggui". Angelica, as a kind of medicinal material with both medicine and food, has a long history of medication and is widely used in clinical practice. [0003] The chemical composition of a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 熊毅邓雪琪李俊饶高雄陈超
Owner 云南昊辰制药有限公司
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