Transcription factor EjCAL participating in regulation and control of flower bud differentiation of loquats and application of transcription factor EjCAL

A transcription factor and flower bud differentiation technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of indistinguishable, the late flowering time is greatly affected, the gene is rare, etc., to solve the young jelly damage and improve the yield of loquat. Effect

Active Publication Date: 2020-07-24
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the level of transcriptional regulation, multiple MDAS-Box family genes have been found in loquat, such as EdFT, EdCO, EdGI, EdSOC1, EdPIF4, EdFD1, EdFD2, EdSVP1, EdSVP1, etc. These genes are all involved in the flowering process of loquat, but, and Studies on the genes related to loquat flower bud differentiation are very rare, and there is no report on the role of EjCAL in regulating the formation and development of loquat flowers
[0004] At present, there is no suitable method to monitor the flower bud differentiation time of loquat, especially at the beginning of flower bud differentiation, which cannot be distinguished by naked eyes, and the variety and environmental factors have a great influence on the flower bud differentiation time and later flowering time, resulting in frequent occurrence of freezing damage to young fruit. Seriously affect the yield and quality of mature fruit

Method used

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  • Transcription factor EjCAL participating in regulation and control of flower bud differentiation of loquats and application of transcription factor EjCAL
  • Transcription factor EjCAL participating in regulation and control of flower bud differentiation of loquats and application of transcription factor EjCAL
  • Transcription factor EjCAL participating in regulation and control of flower bud differentiation of loquats and application of transcription factor EjCAL

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Embodiment 1

[0017] This embodiment is the acquisition of the full-length cDNA sequence of the transcription factor EjCAL:

[0018] S1. Extraction of total RNA from loquat flower buds by improved CTAB method: Weigh 0.5 g of loquat flower buds stored at -80°C, grind them fully in liquid nitrogen, add to 4ml of 65°C preheated CTAB extraction buffer (containing 80 μl of β-mercaptoethanol) in a centrifuge tube, vortexed and heated at 65°C for 2 min; then add 4 ml of chloroform / isoamyl alcohol extract to the centrifuge tube (volume ratio of chloroform and isoamyl alcohol 24:1), vortex mixed, then centrifuged at 4°C and 10,000rpm for 10min, sucked up the supernatant, added 4ml of chloroform / isoamyl alcohol extract, and then re-centrifuged at 10,000rpm Centrifuge for 10min, draw the supernatant, add 1 / 4 volume of LiCl with a concentration of 10mol / l 2 , and then placed at a temperature of 4°C for 8h to 16h, the next day, centrifuged at a temperature of 4°C and a speed of 10,000rpm for 30min, dis...

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Abstract

The invention provides a transcription factor EjCAL participating in regulation and control of flower bud differentiation of loquats. A nucleotide sequence of the transcription factor EjCAL is represented by SEQ ID NO.1, and an amino acid sequence of the transcription factor EjCAL is represented by SEQ ID NO.2. The transcription factor EjCAL provided by the invention has the function of monitoringthe flower bud differentiation of the loquats, can be used for effectively monitoring flower bud differentiation conditions of the loquats according to change of expressive abundance of the loquats and is convenient for subsequent regulation and control of a flowering stage of the loquats, so that the freeze injury problem of the loquats is effectively solved, and the yield of the loquats is increased.

Description

technical field [0001] The invention belongs to the technical field of plant molecular biology, and specifically relates to a transcription factor EjCAL involved in the regulation and control of loquat flower bud differentiation and an application thereof. Background technique [0002] In plant ontogeny, floral differentiation is the beginning of reproductive growth. First, the vegetative growth tissue transforms into the inflorescence meristem, then the inflorescence meristem transforms into the floral meristem, next, the floral meristem gives rise to floral organ primordia, and finally various floral organs. The identified floral meristem-determining genes include LEAFY (LFY), APETALA1 (AP1), CAULIFLOWER (CAL), APETALA2 (AP2), and UNUSUAL FLORAL ORGANS (UFO). Among them, CAL plays an important role in plant flower development. [0003] Loquat (Eriobotrya japonica Lindl.) originated in China and is one of the important fruit trees in southern my country. The physiological...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/158
Inventor 徐红霞陈俊伟李晓颖王朝丽
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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