A kind of Quercus root-promoting microorganism microcapsule inoculum and preparation method thereof

A technology of microorganisms and microcapsules, applied in the field of bio-organic fertilizers, can solve problems such as the degradation of the root-promoting bacteria of growth-promoting bacteria, and achieve the effects of prolonging the storage period, being suitable for large-scale large-scale production, and reducing metabolism

Active Publication Date: 2021-05-18
SHANGHAI CHENSHAN BOTANICAL GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, due to the characteristics of rapid growth, reproduction and rapid variation of microorganisms, the root-promoting performance of growth-promoting bacteria may degrade, and the use of growth-promoting bacteria is also limited by regional, crop types, and environmental conditions. The screening of strains that provide crops with a large amount of trace elements or have growth-promoting functions for crops, such as dissolving potassium, is an important work at present.

Method used

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  • A kind of Quercus root-promoting microorganism microcapsule inoculum and preparation method thereof
  • A kind of Quercus root-promoting microorganism microcapsule inoculum and preparation method thereof
  • A kind of Quercus root-promoting microorganism microcapsule inoculum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of Quercus plant root-promoting microorganism microcapsule inoculant agent, and its components include: 10% of rhizobium radiata liquid, 10% of Bacillus megaterium liquid, 10% of seawater Bacillus liquid, jelly-like spore-like bacteria Bacillus liquid 10%, white carbon black 20%, corn starch 20%, gum arabic 8%, trehalose 4%, skimmed milk powder 3.9%, glycerin 4%, sodium naphthalene acetate 0.1%; The number of effective viable bacteria in the bacillus liquid, seawater bacillus liquid and jelly-like Paenibacillus liquid was 1.38×10 10 cfu / g, the number of miscellaneous bacteria is 3.28%.

[0040] Obtained by the following preparation method:

[0041] 1) Preparation of seed liquid: Rhizobia radiata, Bacillus megaterium, Bacillus marinum, and Paenibacillus jelly-like were respectively inoculated in the corresponding seed medium, cultured on a shaker at 30°C and 200r / min for 12 hours, and prepared into seed liquid ;

[0042] 2) Fermentation: Put the seed liquid pre...

Embodiment 2

[0049] The field application experiment of embodiment 2 microbial microcapsule bacterial agents:

[0050] Select 1m in the planting field for planting A. microphylla, A. spp. 2 There are 12 plots, which are divided into 4 groups on average, and there are 60 seedlings per square meter. Among them, three groups were respectively applied with Qifu EM planting bacterial classification, TMF bacterial agent, and the microbial microcapsule bacterial agent prepared in Example 1, and the remaining group did not apply any bacterial agent as a control. The four groups of sample plots had the same treatment conditions and grew for six months. After that, 10 experimental seedlings were randomly selected in each plot to measure the height and dry biomass of the experimental seedlings, and the root system was scanned, and the root length, surface area, diameter, and number of root tips were counted, and the average was calculated. The results are shown in Table 1.

[0051] Table 1

[0052]...

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Abstract

The invention provides a root-promoting microbial microcapsule agent of Quercus plants and a preparation method thereof, wherein the active ingredients of the microbial microcapsule agent are Rhizobium radiata, Bacillus megaterium, Bacillus seawater, and Paenibacillus jelly-like. The storage time of microbial microcapsule inoculum at room temperature is long, and the survival rate of bacteria is high, which can promote the growth of root length and root tip of Quercus plants; the present invention adopts spray drying technology to prepare microbial microcapsule inoculum, and the design of production process is reasonable to ensure Cleanliness and sterility of the agent.

Description

technical field [0001] The invention belongs to the technical field of bio-organic fertilizers, and in particular relates to a microcapsule agent for root-promoting microorganisms of Quercus plants and a preparation method thereof. Background technique [0002] The broad genus Quercus Fagaceae includes subgenus Cyclobalanopsis and subg Quercus, which are widely distributed in tropical, subtropical and temperate forests in northern hemispheres such as Asia, Africa, Europe and America, and many of them are still There are about 600 main constructive and dominant species in forests in the northern hemisphere, and 127 species in China. In the past, people’s research on Quercus species mainly focused on economic value, such as wood utilization and fruit utilization. In recent years, with the improvement of the quality of urban greening, some native tree species and foreign newly introduced tree species have gradually appeared in urban green spaces. more abundant. At this stage,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/25A01N63/22A01N63/20A01N37/10A01N43/38A01P21/00C12N1/20C12N11/10C12R1/41C12R1/11C12R1/01
CPCA01N37/10A01N43/38A01N63/00C12N1/20C12N11/10
Inventor 张国威商侃侃胡永红秦俊蒋云
Owner SHANGHAI CHENSHAN BOTANICAL GARDEN
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