Gelatin sponge and preparation method and storage method thereof

A gelatin sponge and sponge technology, applied in the field of biomedicine, can solve the problems of proliferation ability, differentiation ability, migration and loss of interaction between cells and extracellular matrix signal transmission, and achieve the effect of improving the clinical compliance rate.

Inactive Publication Date: 2020-08-07
江苏安泰康健康科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a scaffold that can be used as a tumor tissue/or cell three-dimensional structure growth, which solves the biological characteristics of the tumor tissue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, the preparation method of gelatin sponge, it comprises:

[0045] Step S1: pre-treatment of collagen raw material, the collagen raw material is bovine deep flexor tendon;

[0046]Step S2: enzymatic hydrolysis of collagen raw materials to obtain bovine deep flexor tendon fiber collagen;

[0047] Step S3: salting out and purifying collagen to purify bovine deep flexor tendon fiber collagen;

[0048] Step S4: homogenizing bovine deep flexor tendon fiber collagen;

[0049] Step S5: preparing a gelatin mixture;

[0050] Step S6, storing the gelatin mixture.

[0051] In step S1, pretreatment of collagen raw materials, that is, pretreatment of bovine deep flexor tendon, including: removing fascia, fat, muscle and other surface non-collagen and other irrelevant substances in bovine deep flexor tendon; washing with tap water and freezing The bovine deep flexor tendon is cut into slices with a thickness of about 0.4-1mm along the longitudinal axis perpendicular t...

Embodiment 2

[0064] Example 2, gelatin sponge was prepared by coating sponge sheet with gelatin.

[0065] The present invention also provides a preparation method of a gelatin sponge supporting three-dimensional growth of tumor tissue / or cells, which is prepared by coating a sponge sheet with a gelatin mixture, and the preparation method comprises:

[0066] Step S10: preparing a gelatin mixture, the gelatin mixture comprising 1-2% bovine deep flexor tendon fiber collagen, 2-5% cyanic acid, 50-70V / V% formic acid and 30-50V / V% Tris distilled water;

[0067] Step S20: adding the gelatin mixture onto the sponge sheet;

[0068] Step S30: drying the sponge sheet after adding the gelatin mixture at 36-40°C overnight, so that the solvent in the gelatin mixture evaporates, so that the bovine flexor tendon fiber collagen aggregates, deposits and adheres to the sponge sheet to obtain The gelatin sponge.

[0069] In a preferred embodiment, in step S10, the gelatin mixture comprises: mass fraction 2...

Embodiment 3

[0073] A gelatin sponge that supports three-dimensional growth of tumor tissue / or cells prepared by the preparation method in Example 1 or Example 2 is provided.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses gelatin sponge as well as a preparation method and a storage method thereof. The preparation method comprises the following steps: mixing bovine flexor tendon fiber collagen with a mixture of cyanic acid and methanol, and homogenizing by virtue of a pore pump, so as to prepare a gelatin mixture; and adhering the gelatin mixture and sponge to form the gelatin sponge.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a gelatin sponge with high biocompatibility capable of supporting three-dimensional growth of tumor tissue / or cells, a preparation method and a storage method. Background technique [0002] In vitro three-dimensional culture is an experimental technique for co-culturing cells, growth factors and reconstituted matrix proteins, and has been widely used in various fields of biomedical research. As a bridge between in vitro cell-free system and single-layer cell system research and tissue organ and overall research, it can not only retain the material and structural basis of the cell microenvironment in vivo, but also demonstrate the intuitiveness and condition controllability of cell culture. Advantage. Current studies have found that human tumor cells cultured in 3D are significantly different from tumor cells cultured in 2D in terms of biological characteristics, gene express...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08J9/42C12N5/09C08L1/02C08L89/00
CPCC08J9/42C08J2301/02C08J2489/00C12N5/0693C12N2513/00C12N2533/54
Inventor 孙晓娇张红霞刘星陆重益张文平
Owner 江苏安泰康健康科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap