Lateral antibody chip for detecting three mycotoxins by using marker-guided universal probe

A mycotoxin and antibody chip technology, used in biological testing, measuring devices, material inspection products, etc., can solve probe mutual interference, misjudgment, orderly release of immune chip signal probes, specific identification and complex background control, etc. It can improve the detection signal intensity and sensitivity, improve the detection sensitivity and quantitative precision, and achieve the effect of good hydrophilicity.

Inactive Publication Date: 2020-08-21
SHAOGUAN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The orderly release, specific recognition and background control of immune chip signal probes are relatively complex issues, and mutual interference between probes is prone to cause misjudgment of results

Method used

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  • Lateral antibody chip for detecting three mycotoxins by using marker-guided universal probe
  • Lateral antibody chip for detecting three mycotoxins by using marker-guided universal probe
  • Lateral antibody chip for detecting three mycotoxins by using marker-guided universal probe

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A label-guided universal probe for detecting three kinds of mycotoxins lateral antibody chip, including a liner 5, and bifunctional antigen release pads 1 that are sequentially adhered to the liner 5 and partially overlapped between adjacent ones, universal Fluorescent probe release pad 2, cellulose acetate membrane 3, and water-absorbing pad 4; the bifunctional antigen release pad contains a detection antigen formed by coupling ovalbumin with AFB1, OTA, and ZEN, respectively. - Amino-4-hydroxyl-2-naphthalenesulfonic acid" label 62; the universal fluorescent probe release pad comprises a universal fluorescent probe labeled with an anti-7-amino-4-hydroxyl-2-naphthalenesulfonic acid antibody 65 Needle, the fluorescent probe is a quantum dot 61; the cellulose acetate membrane 3 is provided with detection line A, detection line B, detection line C and quality control line 34, the detection line A, detection line B, detection line C respectively immobilize anti-AFB1 antibody...

Embodiment 2

[0056] Assay method for simultaneous detection of AFB1, OTA, and ZEN, see Figure 6 ,Proceed as follows:

[0057] Take 5g of crushed cereal crop samples, fully extract the sample in 25mL of 70% methanol-water solution, centrifuge at 5000rpm for 10 minutes, take 2.0mL of the supernatant and add it to 8.0mL of PBS buffer, shake well to become the sample solution; use a dropper Add 3-4 drops of sample solution on the bifunctional antigen release pad. During the movement of the sample solution to the nitrocellulose membrane, the bifunctional antigen and the universal fluorescent probe are released, and successively cross the detection line and the quality control line. According to the detection line A, detection line B, detection line C and the fluorescent signal on the quality control line to determine whether the sample contains AFB1, OTA and ZEN.

[0058] refer to Figure 5 . The AFB1 antibody 311 immobilized on the nitrocellulose membrane detection line A, and the anti-7-a...

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Abstract

The invention relates to a lateral antibody chip for detecting three mycotoxins by using a label-guided universal probe. The lateral antibody chip comprises a lining plate, and a difunctional antigenrelease pad, a universal fluorescent probe release pad, a chromatographic membrane and a water absorption pad which are sequentially adhered to the lining plate and are partially overlapped with one another, wherein the bifunctional antigen release pad comprises a specific marker coupled on the basis of a detection antigen formed by respectively coupling ovalbumin with AFB1, OTA and ZEN; the universal fluorescent probe release pad comprises a universal fluorescent probe labeled with an anti-specific marker antibody; the chromatographic membrane is provided with detection lines A, B and C and aquality control line, the detection lines A, B and C are respectively used for fixing an anti-AFB1 antibody, an anti-OTA antibody and an anti-ZEN antibody, and the quality control line is used for fixing the anti-specific marker antibody. According to the invention, the production of all detection reagents can be solved by using the universal fluorescent probe, the cost is reduced, and the quality control reliability is improved; and the signal intensity and the stability of detection are improved, and the detection sensitivity and the quantitative precision are improved.

Description

technical field [0001] The invention relates to the field of food and drug safety detection, in particular to a lateral antibody chip for detecting three mycotoxins with marker-guided universal probes. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi. At present, more than 300 mycotoxins with different chemical structures are known, among which there are more than 30 mycotoxins produced by fungal strains, which are carcinogenic, genotoxic, and teratogenic to humans and animals. It can cause hepatotoxicity, nephrotoxicity, reproductive abnormalities and suppress immune response, which is extremely toxic and harmful. [0003] The possibility that agricultural products may be contaminated by a variety of fungi, which metabolize to produce mixed contamination of a variety of mycotoxins, is attracting worldwide attention. Authoritative studies at home and abroad have shown that peanuts, corn, soybeans and other grain and oil crops may be simul...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N33/58G01N33/558G01N33/53
CPCG01N33/582G01N33/588G01N33/558G01N33/5308
Inventor郭杰标
OwnerSHAOGUAN COLLEGE