A semen freeze-dilution method capable of automatically adding liquid and adjusting the height of pre-freezing

A cryo-dilution and pre-freezing technology, which is applied to the preservation of human or animal bodies, animal delivery, medical science, etc., can solve the problems of insufficient attention, large differences in dilution effects, and unscientific problems, so as to improve the quality of frozen semen and reduce Probability of inactivation, effect of avoiding dilution effects

Active Publication Date: 2021-12-24
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Abstract
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AI Technical Summary

Problems solved by technology

The speed of adding diluent and the mixing speed of semen and diluent have a key influence on the quality of frozen semen, but for a long time, researchers and daily production personnel have not paid enough attention to this link, and semen dilution is basically manual, relying on experience The operation, diluent adding speed and the speed of mixing semen and diluent cannot be standardized, and the dilution effect of different operators varies greatly, which is not uniform and standard, which is one of the important reasons for the quality difference of different batches of frozen semen
[0004] In addition, during the liquid nitrogen gas phase prefreezing operation, the current prefreezing device is a metal pedestal, and the thin tube is placed on the metal pedestal and then put into the liquid nitrogen gas phase for prefreezing. However, for a long time, researchers and daily production personnel have not I didn’t realize that the temperature of the liquid nitrogen gas phase varies with different heights. Different species of animals require different temperatures for pre-freezing semen. If no matter what animal’s semen is pre-frozen, only one metal pedestal, regardless of the height of the frozen semen tube from the liquid nitrogen surface, this is not scientific, and it has not fully tapped the potential to improve the quality of frozen semen

Method used

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  • A semen freeze-dilution method capable of automatically adding liquid and adjusting the height of pre-freezing
  • A semen freeze-dilution method capable of automatically adding liquid and adjusting the height of pre-freezing
  • A semen freeze-dilution method capable of automatically adding liquid and adjusting the height of pre-freezing

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Contrastive experiment of manual diluent addition and automatic diluent addition on the quality of sheep frozen semen

[0034] The semen of 10 adult Yunnan semi-fine wool sheep rams was collected by electrical stimulation, and sent to the laboratory for fresh semen quality inspection. Semen motility above 75%, sperm density at 2.5×10 9 / ml, and the semen with a deformity rate below 10% is used for cryopreservation. Mix the semen that meets the standards evenly to eliminate the differences between individuals, then divide the semen into 12 parts on average, select 3 operators, and each person will manually add diluent to 3 parts of the semen, and the laboratory has prepared 3 sets The automatic liquid addition and dilution device automatically adds diluent to the other 3 semen, and the specific groups are as follows: operator 1 (conventional manual dilution), operator 2 (conventional manual operation), operator 3 (conventional manual dilution), Groups were ad...

Embodiment 2

[0039] Example 2 The effect of the height of the pre-freezing device on the vigor of frozen semen of sheep

[0040] The semen of 5 adult Yunnan semi-fine wool sheep rams were collected by electrical stimulation, and sent to the laboratory for fresh semen quality inspection. Semen motility above 75%, sperm density at 2.5×10 9 / ml, and the semen with a deformity rate below 10% is used for cryopreservation. The automatic liquid-adding and diluting device of the present invention is assembled in advance for use, and the dilution conditions are as follows: the dilution rate is 50 microliters / second + the shaker speed is 50 rpm, the shaker temperature is 38° C., and the semen density after final dilution is 4.0×10 8 / ml. The diluted semen was divided into 0.25ml plastic thin tubes, and after sealing, the temperature was slowly lowered to 5°C and kept at this temperature for 4 hours. The test group was divided into 4 groups, and the distance between the metal mesh of the pre-freez...

Embodiment 3

[0044] Example 3 The influence of the height of the pre-freezing device on the viability of goat frozen semen

[0045] The semen of 5 adult Yunshang black goat rams was collected by the false vagina method, and sent to the laboratory for fresh semen quality inspection. Semen motility above 75%, sperm density at 2.5×10 9 / ml, and the semen with a deformity rate below 10% is used for cryopreservation. The automatic liquid addition and dilution device of the present invention is assembled in advance for use, and the dilution conditions are as follows: the dilution rate is 80 microliters / second + the shaker speed is 60 rpm, and the shaker temperature is set at 38°C. The diluted semen density is 4.0×10 8 / ml. The diluted semen was divided into 0.25ml plastic thin tubes, and after sealing, the temperature was slowly lowered to 5°C and kept at this temperature for 4 hours. The test group was divided into 4 groups, and the distance between the metal mesh of the pre-freezing device...

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Abstract

The invention relates to a semen freeze-dilution method capable of automatically adding liquid and adjusting the height of pre-freezing, comprising the following steps: putting fresh semen into a 50ml beaker, preheating on a constant temperature water bath shaker of an automatic liquid-adding and diluting device, and shaking the beaker The rotational speed is set at 10-220rpm; the speed of diluent dripping is controlled at 10-200 microliters per second; during liquid nitrogen gas-phase pre-freezing, the height of the pre-freezing device is determined according to the animal species, and finally put into liquid nitrogen for storage. The invention can automatically add diluent, standardize the operation, eliminate the interference of personal manual operation, and adjust different liquid nitrogen gas-phase pre-freezing heights according to different animals. The device used in the method of the present invention has a simple structure and can be used by simply modifying the existing equipment in the laboratory, so it will not affect the price of frozen semen, but the quality of frozen semen is significantly improved, and lambing can be improved in production tests. The rate is 8‑9 percentage points, which significantly improves the production efficiency of livestock farms.

Description

technical field [0001] The invention relates to the field of animal reproduction and breeding, in particular to a semen freeze-dilution method capable of automatically adding liquid and adjusting the pre-freezing height. Background technique [0002] In modern animal husbandry, most cattle and pigs are raised with frozen semen for artificial insemination to impregnate female animals. Bulls are rarely raised in farms. The frozen semen must be diluted and made into thin tubes. One thin tube for each female animal. Male animals can impregnate many female animals. Semen cryopreservation technology is of great significance for the protection of genetic resources and the promotion of artificial insemination technology. The quality of frozen semen is related to the efficiency of pregnancy and production of female animals and the strength of offspring, and is a key factor affecting the production efficiency of modern animal husbandry. Usually, the semen freezing process includes se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02A61D19/02
CPCA01N1/0252A61D19/025
Inventor 权国波吕春荣梁家充吴国权邵庆勇信爱国洪琼花
Owner YUNNAN ANIMAL SCI & VETERINARY INST
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