Molecular marker primer group based on camellia oleifera transcriptome hAT transposon and application thereof

A technology of molecular markers and transcriptomes, applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., to achieve good polymorphism effect

Active Publication Date: 2020-09-01
BEIBU GULF UNIV
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Problems solved by technology

[0004] At present, no application of hAT transposon in camellia oleifera has been found, so an in-depth understanding of transposon will be of great importance to promote the development of theory and technology of camellia oleifera genetics and breeding. has a very important role

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  • Molecular marker primer group based on camellia oleifera transcriptome hAT transposon and application thereof
  • Molecular marker primer group based on camellia oleifera transcriptome hAT transposon and application thereof
  • Molecular marker primer group based on camellia oleifera transcriptome hAT transposon and application thereof

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Embodiment 1

[0027] 1. Sample:

[0028] The samples used in this experiment are all from Guangxi, among which the common Camellia came from Sanmenjiang Forest Farm in Liuzhou, Guangxi, and the Vietnamese Camellia came from Pingxiang County and Ningming County in Chongzuo, Guangxi, which are marked as: Honghu Subfield-Taohuadao (T), Ma Santangtun (S) of Dongcun Village and Nongzhangtun (N) of Madong Village. Fragrant Flower Camellia comes from Nanning, Guangxi and is labeled XH. A total of 109 representative varieties of Camellia oleifera were selected for analysis, including 25 common camellias, 42 Vietnamese camellias, and 42 fragrant flower camellias.

[0029] Collection method: Pick 1-2 fresh leaves from the Camellia oleifera tree by manual picking, put them in ziplock bags, and mark them with corresponding numbers. Store in a -20°C refrigerator in the laboratory. In this experiment, the fresh leaves taken will be used as test materials for extracting Camellia oleifera genomic DNA. ...

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Abstract

The invention relates to the technical field of molecular markers, in particular to a molecular marker primer group based on a camellia oleifera transcriptome hAT transposon and an application thereof. The primer disclosed by the invention is designed aiming at a camellia oleifera transcriptome hAT transposon; seven pairs of designed primers have relatively high polymorphic information content (PIC); good polymorphism is realized; the camellia oleifera varieties can be well distinguished; according to the application, the feasibility of developing the hAT transposon marker by utilizing commoncamellia oleifera is proved; genetic diversity analysis is carried out on common oil-tea camellia, Vietnamese oil-tea camellia and fragrant flower oil-tea camellia, so that novel high-quality molecular markers can be provided for the oil-tea camellia, a good foundation is laid for molecular improvement of the oil-tea camellia, and new primers can be provided for research on genetic breeding, genome mapping, gene localization, species genetic relationship identification and the like in the future.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular markers, in particular to a molecular marker primer set based on the camellia oleifera transcriptome hAT transposon and its application. 【Background technique】 [0002] Camellia oleifera is mainly planted in the subtropical regions of the south, and is widely distributed in the hilly areas of 17 provinces in the south. Guangxi's camellia oleifera planting area, annual output and total industrial output value rank third in the country. Camellia oleifera is planted in a wide range and a large area in my country. However, the yield has been low for a long time. The reasons include few improved varieties, low levels, disordered germplasm resources, etc. The root cause is the lack of improved varieties. Although many remarkable achievements have been made under the long-term joint efforts of Chinese researchers, there are still many problems in the improvement of Camellia oleifera varieties. Gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6806C12N15/11
CPCC12Q1/6895C12Q1/6806C12Q2600/156C12Q2531/113
Inventor 王鹏良张照远杨利平谢雅榆吴双成叶航苏洁霞
Owner BEIBU GULF UNIV
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