Molecular marker primer set based on hat transposon in camellia oleifera transcriptome and its application

A technology of molecular markers and primer sets, applied in the direction of recombinant DNA technology, biochemical equipment and methods, microbial determination/testing, etc.

Active Publication Date: 2022-06-14
BEIBU GULF UNIV
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  • Claims
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Problems solved by technology

[0004] At present, no application of hAT transposon in camellia oleifera has been found, so an in-depth understanding of transposon will be of great importance to promote the development of theory and technology of camellia oleifera genetics and breeding. has a very important role

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  • Molecular marker primer set based on hat transposon in camellia oleifera transcriptome and its application
  • Molecular marker primer set based on hat transposon in camellia oleifera transcriptome and its application
  • Molecular marker primer set based on hat transposon in camellia oleifera transcriptome and its application

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Embodiment 1

[0027] 1. Sample:

[0028] The samples used in this experiment are all from Guangxi, among which the common Camellia oleifera comes from Sanmenjiang Forest Farm in Liuzhou, Guangxi, and the Vietnamese Camellia oleifera comes from Pingxiang County and Ningming County in Chongzuo, Guangxi. Santangtun (S) of Dongcun Village and Nongzhangtun (N) of Madong Village. Fragrant Flower Camellia comes from Nanning, Guangxi and is labeled XH. A total of 109 representative varieties of Camellia oleifera were selected for analysis, including 25 common camellias, 42 Vietnamese camellias, and 42 fragrant flower camellias.

[0029] Collection method: Pick 1-2 fresh leaves from the Camellia oleifera tree by manual picking, put them in ziplock bags, and mark them with corresponding numbers. Store in a -20°C refrigerator in the laboratory. In this experiment, the fresh leaves taken will be used as test materials for extracting Camellia oleifera genomic DNA.

[0030] 2. Experimental equipment:...

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Abstract

The invention relates to the technical field of molecular markers, in particular to a molecular marker primer set based on the hAT transposon of the Camellia oleifera transcriptome and its application. The primers of the present invention are designed for the hAT transposon of the Camellia oleifera transcriptome. Higher polymorphic information content (PIC), good polymorphism, and good differentiation of Camellia oleifera varieties. This application confirms the feasibility of using common Camellia oleifera to develop hAT transposon markers, and the common Camellia oleifera, Camellia oleifera The genetic diversity analysis of Camellia oleifera and Camellia oleifera can not only provide novel and high-quality molecular markers for Camellia oleifera, but also lay a good foundation for molecular improvement of Camellia oleifera, which can be used for future genetic breeding, genome mapping, gene positioning and species kinship. New primers are provided for researches such as relationship identification.

Description

【Technical field】 [0001] The invention relates to the technical field of molecular markers, in particular to a molecular marker primer set based on the camellia oleifera transcriptome hAT transposon and its application. 【Background technique】 [0002] Camellia oleifera is mainly planted in the subtropical regions of the south, and is widely distributed in the hilly areas of 17 provinces in the south. Guangxi's camellia oleifera planting area, annual output and total industrial output value rank third in the country. Camellia oleifera is planted in a wide range and a large area in my country. However, the yield has been low for a long time. The reasons include few improved varieties, low levels, disordered germplasm resources, etc. The root cause is the lack of improved varieties. Although many remarkable achievements have been made under the long-term joint efforts of Chinese researchers, there are still many problems in the improvement of Camellia oleifera varieties. Gene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6806C12N15/11
CPCC12Q1/6895C12Q1/6806C12Q2600/156C12Q2531/113
Inventor 王鹏良张照远杨利平谢雅榆吴双成叶航苏洁霞
Owner BEIBU GULF UNIV
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