A kind of virus sampling liquid composition and sampling method

A composition and virus technology, applied in the direction of recombinant DNA technology, DNA preparation, etc., can solve problems such as clinical diagnosis errors, inaccurate experimental data, and loss of farms in farms, and achieve prolonged survival time, high sampling efficiency, and guaranteed The effect of accuracy

Active Publication Date: 2022-08-05
福建省辉腾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are following shortcomings in the current sampling method of pig herd virus: (1) generally only have sampling equipment for pig herd at present, generally be the equipment that special pig herd environment is set and sampled; (2) the collected sample is due to Due to various reasons such as storage and time difference, the nucleic acid in the sample is easy to degrade, resulting in inaccurate experimental data, making the farm make wrong judgments in clinical diagnosis, which brings great losses to the farm

Method used

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  • A kind of virus sampling liquid composition and sampling method
  • A kind of virus sampling liquid composition and sampling method
  • A kind of virus sampling liquid composition and sampling method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kind of veterinary sampling liquid composition, it comprises sampling liquid and preservation liquid, and described sampling liquid comprises the following components of parts by weight:

[0033]

[0034] The preservation solution includes the following components by weight:

[0035]

[0036] After each component of the sampling liquid is mixed uniformly, a sealed bag is filled by every 40-50 parts by weight; after each component of the preservation solution is mixed uniformly, a sealed bag is filled by every 2-3 parts by weight.

Embodiment 2

[0038] A kind of veterinary sampling liquid composition, it comprises sampling liquid and preservation liquid, and described sampling liquid comprises the following components of parts by weight:

[0039]

[0040]

[0041] The preservation solution includes the following components by weight:

[0042]

[0043] After each component of the sampling liquid is mixed uniformly, a sealed bag is filled by every 40-50 parts by weight; after each component of the preservation solution is mixed uniformly, a sealed bag is filled by every 2-3 parts by weight.

Embodiment 3

[0045] A kind of veterinary sampling liquid composition, it comprises sampling liquid and preservation liquid, and described sampling liquid comprises the following components of parts by weight:

[0046]

[0047] The preservation solution includes the following components by weight:

[0048]

[0049] After each component of the sampling liquid is mixed uniformly, a sealed bag is filled by every 40-50 parts by weight; after each component of the preservation solution is mixed uniformly, a sealed bag is filled by every 2-3 parts by weight.

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Abstract

The invention discloses a virus sampling liquid composition, which includes a sampling liquid and a preservation liquid. The sampling liquid includes the following components: sodium chloride, disodium hydrogen sulfate, potassium dihydrogen sulfate, potassium chloride, water, phenol red ; The preservation solution includes the following components: glucose, trehalose, wolfberry polysaccharide, aloe vera polysaccharide, thiomersal sodium salt, and water. Trehalose, Lycium barbarum polysaccharide and aloe vera polysaccharide are added to the preservation solution of the present invention, which can greatly slow down the degradation rate of nucleic acid in saliva samples, prolong the survival time of viruses in the samples, and ensure the accuracy of detection. At the same time, the present invention is specially equipped with a sampling liquid. The sampling liquid is used to fully infiltrate the sampling tool before sampling. Due to the lubricating effect of the sampling liquid, the adhesion amount of the virus can be increased and the sampling efficiency can be improved. In addition, the sampling method of the present invention has simple operation, convenient sampling and high sampling efficiency.

Description

technical field [0001] The invention relates to the field of virus inspection and quarantine, in particular to a virus sampling liquid composition and a sampling method. Background technique [0002] Viruses are non-cellular parasitic living organisms composed of a nucleic acid molecule (DNA or RNA) and proteins. Viruses are highly parasitic and completely depend on the energy and metabolic systems of host cells to obtain substances and energy required for life activities. Humans have recorded viral infections as early as 1400 BC, but viruses were not gradually discovered and identified until the end of the 19th century. Viruses can cause various diseases of animals, plants and humans, posing a great threat to human survival and health, such as human immunodeficiency virus, Ebola virus, SARS, H1N1, etc., all of which pose a great threat to humans and animals. . [0003] The detection of virus infection depends on methods such as virus isolation and identification, virus g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
CPCC12N15/10
Inventor 潘津王荣魏俊祥阮雨红张杰
Owner 福建省辉腾生物科技有限公司
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