A preventive treatment method for kelp albinism

A treatment method, a technology for albinism, applied in horticultural methods, botanical equipment and methods, seaweed cultivation, etc., can solve problems such as poor effect, affecting the supply of healthy kelp seedlings, breeding and production, etc.

Active Publication Date: 2022-03-04
OCEAN UNIV OF CHINA
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main symptom is that in the later stage of seedling cultivation, with the increase of light intensity, the tip of the kelp seedlings (tissues far away from the growth point of the base) will produce white spots. If it is not well controlled, green rot will occur from the diseased part. The rot disease spreads to the entire seedling leaves, which greatly affects the supply of healthy kelp seedlings and subsequent breeding production
[0003] Once albino seedlings are found in the kelp seedling stage, the main treatment method is to reduce the temperature, light intensity and increase the number of water changes in the seedling cultivation workshop to inhibit the reproduction of pathogenic bacteria and reduce the number of pathogenic bacteria, but the effect is not good. it is good
At present, there are no very effective measures and methods to prevent and control the outbreak of kelp albinism

Method used

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  • A preventive treatment method for kelp albinism
  • A preventive treatment method for kelp albinism
  • A preventive treatment method for kelp albinism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: The influence of light on the growth of pathogenic bacteria Pseudoalteromonas X-8

[0018] The applicant firstly conducted an experiment on the pathogenic bacterium Pseudoalteromonas X-8 of kelp albinism.

[0019] Among them, the pathogenic bacteria Pseudoalteromonas X-8 was screened from albino kelp seedlings in a kelp nursery in Rongcheng, Shandong Province in December 2017. The counterstaining experiment proved that this strain can cause albinism in healthy kelp seedlings.

[0020] Cultured in Zobell 2216E medium, when X-8 grew to exponential phase, the absorbance of bacterial growth was measured at OD 600 (optical density at 600 nm) using a UV-6000 UV-Vis spectrophotometer (METASH). Purchase white light, blue light (400-450nm) and red light (620-760nm) two monochromatic LED light sources at Ningbo Jiangnan Instrument Factory. Set the light intensity of white light, blue light and red light to 40 μmol m, respectively -2 ·s -1 , 60μmol·m -2 ·s -1 ,,...

Embodiment 2

[0021] Example 2: Cluster Motility Analysis

[0022] Pseudoalteromonas X-8 was grown to logarithmic phase at 25°C in the dark. Subsequently, the bacterial solution was centrifuged at 6000 g for 10 minutes at room temperature to obtain a cell pellet homogenate. Use an inoculating loop to inoculate the bacterial pellet onto the bacterial solid medium. Incubate under blue light, red light, white light and dark conditions for 24 hours at a temperature of 10±1°C. Three replicates were designed for each group of experiments. The light intensity is 80μmol·m -2 ·s -1 . It was found that at 80 μmol·m -2 ·s -1 Under the conditions of , white light and blue light inhibited the movement of Pseudomonas X-8, while red light and darkness promoted the movement of Pseudomonas X-8.

Embodiment 3

[0023] Embodiment 3: Pathogen-kelp counterstaining experiment

[0024] Take out the preserved bacterial liquid from the -20°C refrigerator, absorb 1 mL into 100 mL Zobell 2216E liquid medium and cultivate in a shaking incubator. After 24 hours, absorb 1 mL into 100 mL Zobell 2216E liquid medium to continue culturing. After 12 hours, absorb 2 mL of overnight culture solution Continue to culture in 200mL Zobell 2216E liquid medium, the culture condition is 25°C, and the rotation speed is 120r / min. After 4 hours, draw the bacterial solution to measure the concentration of the bacterial solution; at this time, the growth of the bacterial solution is in the logarithmic growth phase.

[0025] Select healthy and well-growth kelp seedlings, place them in a petri dish filled with sterile seawater, and then cut the sporophytes into small pieces (1.0cm x 1.0cm) with a sterilized blade. Put the kelp tissue block into the culture plate, and add 1mL pseudoalteromonas X-8 bacteria solution ...

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Abstract

The invention provides a method for preventing and treating kelp albinism, which uses blue light or white light to inhibit the pathogenic bacteria of kelp albinism; the light intensity of the white light is less than 40 μmol·m ‑2 ·s ‑1 or greater than 160μmol·m ‑2 ·s ‑1 ; As for the blue light, its light intensity is not lower than 40 μmol·m ‑2 ·s ‑1 . The kelp albinism pathogenic bacteria is Pseudoalteromonas X-8 strain (Pesudoalteromonas X-8), and the preservation number is CGMCC20099. The method of the invention can provide a theoretical basis for kelp seedling raising enterprises to formulate correct and appropriate light intensity management, and provide light intensity parameters for screening albino-resistant kelp strains.

Description

technical field [0001] The invention belongs to the technical field of seaweed disease prevention and control, and in particular relates to a method for preventing and treating kelp albinism. Background technique [0002] Kelp belongs to the genus Kelp of Phaeophyta, which is a large brown algae and a perennial large edible algae. Shandong, Fujian and Liaoning are the main producing areas of kelp in my country, accounting for more than 90% of the national total output. Like crops, economic crops kelp will also suffer from serious diseases during the seedling period. In recent years, albinism is a disease that often occurs in the late stage of kelp seedling cultivation (one and a half months or two months after the initiation of seedling cultivation). The main symptom is that in the later stage of seedling cultivation, with the increase of light intensity, the tip of the kelp seedlings (tissues far away from the growth point of the base) will produce white spots. If it is n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00A01G13/00A01G7/04
CPCA01G33/00A01G13/00A01G7/045
Inventor 王高歌韩琦张晓阳
Owner OCEAN UNIV OF CHINA
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