Short-period efficient all-male pelodiscus sinensis fry breeding method
A Chinese soft-shelled turtle, short-cycle technology, applied in biochemical equipment and methods, microbial determination/inspection, animal husbandry, etc., can solve the problem of inability to identify large quantities of Chinese soft-shelled turtle samples, complex identification methods for pseudo-female soft-shelled turtles, and lack of full males. Chinese soft-shelled turtle and other problems, to achieve the effect of significant economic effect, shortened breeding cycle and good quality
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Embodiment 1
[0045] Embodiment 1: temperature-controlled hatching of soft-shelled turtle eggs
[0046] Take 120 fertilized soft-shelled turtle eggs and place them in a single layer in the incubator. In the incubator, lay about 5cm thick vermiculite in advance, adjust the animal pole of the turtle eggs upward, and continue to cover the vermiculite on the turtle eggs. The stone covering thickness is about 1cm. The incubator is placed in a greenhouse with a temperature of 28±1°C and an air humidity of 80-85%. The humidity of the vermiculite in the incubator is kept at 8-12%. The development of the soft-shelled turtle embryo is observed daily.
Embodiment 2
[0047] Embodiment 2: Soft-shelled turtle egg estrogen treatment
[0048] Taking 60 random soft-shelled turtle eggs in the incubator of Example 1 as the treatment group, when the embryos in the soft-shelled turtle eggs hatched to the 15th stage, estrogen treatment was started. The estrogen is a 17β-estradiol solution with a final concentration of 50 μg / μL prepared in 95 wt% ethanol as a solvent. The distribution position of the soft-shelled soft-shelled turtle embryo in the soft-shelled turtle egg is determined using an egg canister, and the eggshell surface closest to the embryo position Add 5 μL of 17β-estradiol solution dropwise. After the addition is complete, put the soft-shelled turtle eggs back to the original position to continue hatching and observe daily. For soft-shelled soft-shelled turtle eggs hatched to the 15-18 stage, drug treatment was carried out once at each stage, and the position and dosage of each dripping method were unchanged, and a total of four estroge...
Embodiment 3
[0052] Embodiment 3: juvenile soft-shelled turtle culture in greenhouse
[0053] About one week after the hatching of the soft-shelled soft-shelled turtle eggs, the juvenile soft-shelled turtles are transferred to the adult turtle breeding pool in the greenhouse. At 5 o'clock, the amount of feed fed each time is about 5-7% of the current juvenile soft-shelled turtle's body weight. When it is about 1 year old, the juvenile soft-shelled turtle is sexually mature.
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