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Short-period efficient all-male pelodiscus sinensis fry breeding method

A Chinese soft-shelled turtle, short-cycle technology, applied in biochemical equipment and methods, microbial determination/inspection, animal husbandry, etc., can solve the problem of inability to identify large quantities of Chinese soft-shelled turtle samples, complex identification methods for pseudo-female soft-shelled turtles, and lack of full males. Chinese soft-shelled turtle and other problems, to achieve the effect of significant economic effect, shortened breeding cycle and good quality

Inactive Publication Date: 2020-10-02
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is a lack of economical and efficient all-male soft-shelled soft-shelled turtle breeding technology, especially the artificial induction method for pseudo-female soft-shelled soft-shelled turtle is relatively complicated, and the transformation rate is low; death, and cannot be applied to the identification of large quantities of soft-shelled turtle samples

Method used

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  • Short-period efficient all-male pelodiscus sinensis fry breeding method
  • Short-period efficient all-male pelodiscus sinensis fry breeding method
  • Short-period efficient all-male pelodiscus sinensis fry breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: temperature-controlled hatching of soft-shelled turtle eggs

[0046] Take 120 fertilized soft-shelled turtle eggs and place them in a single layer in the incubator. In the incubator, lay about 5cm thick vermiculite in advance, adjust the animal pole of the turtle eggs upward, and continue to cover the vermiculite on the turtle eggs. The stone covering thickness is about 1cm. The incubator is placed in a greenhouse with a temperature of 28±1°C and an air humidity of 80-85%. The humidity of the vermiculite in the incubator is kept at 8-12%. The development of the soft-shelled turtle embryo is observed daily.

Embodiment 2

[0047] Embodiment 2: Soft-shelled turtle egg estrogen treatment

[0048] Taking 60 random soft-shelled turtle eggs in the incubator of Example 1 as the treatment group, when the embryos in the soft-shelled turtle eggs hatched to the 15th stage, estrogen treatment was started. The estrogen is a 17β-estradiol solution with a final concentration of 50 μg / μL prepared in 95 wt% ethanol as a solvent. The distribution position of the soft-shelled soft-shelled turtle embryo in the soft-shelled turtle egg is determined using an egg canister, and the eggshell surface closest to the embryo position Add 5 μL of 17β-estradiol solution dropwise. After the addition is complete, put the soft-shelled turtle eggs back to the original position to continue hatching and observe daily. For soft-shelled soft-shelled turtle eggs hatched to the 15-18 stage, drug treatment was carried out once at each stage, and the position and dosage of each dripping method were unchanged, and a total of four estroge...

Embodiment 3

[0052] Embodiment 3: juvenile soft-shelled turtle culture in greenhouse

[0053] About one week after the hatching of the soft-shelled soft-shelled turtle eggs, the juvenile soft-shelled turtles are transferred to the adult turtle breeding pool in the greenhouse. At 5 o'clock, the amount of feed fed each time is about 5-7% of the current juvenile soft-shelled turtle's body weight. When it is about 1 year old, the juvenile soft-shelled turtle is sexually mature.

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Abstract

The invention relates to an all-male pelodiscus sinensis fry breeding technology, in particular to a short-period efficient all-male pelodiscus sinensis fry breeding method. The method comprises the steps of pelodiscus sinensis egg temperature control hatching, pelodiscus sinensis egg estrogen treatment, young pelodiscus sinensis greenhouse breeding, pseudo-female pelodiscus sinensis screening andparent pelodiscus sinensis pond breeding, wherein the pelodiscus sinensis egg hatching temperature is controlled to be 28+ / -1 DEG C in the pelodiscus sinensis egg temperature control hatching; the pelodiscus sinensis egg estrogen treatment is that an estradiol solution is dropwise added to the eggshell surface closest to the embryo position in each of 15-18 time periods of pelodiscus sinensis embryo hatching; and a fluorescent quantitative PCR technology is adopted in the pseudo-female pelodiscus sinensis screening. According to the method, a sex hormone dropwise adding and pseudo-female pelodiscus sinensis induction technology is adopted, the operation is easy, the conversion rate is high, the sexual maturity period is greatly shortened through the young pelodiscus sinensis greenhouse breeding, and pseudo-female pelodiscus sinensis screened out through the fluorescent quantitative PCR technology are normally mated with original male pelodiscus sinensis to obtain all-male pelodiscus sinensis fries. The all-male pelodiscus sinensis fry breeding method provided by the invention is short in period and high in commercialization degree, and economic benefits can be remarkably improved.

Description

technical field [0001] The invention relates to the breeding technology of all male soft-shelled soft-shelled turtles, in particular to a short-period high-efficiency breeding method for all male Chinese soft-shelled turtles. [0002] technical background [0003] Chinese soft-shelled turtle (Pelodiscus sinensis), commonly known as soft-shelled turtle, is an important and valuable aquaculture species in my country. The male soft-shelled soft-shelled turtle grows faster than the female soft-shelled turtle, has a wider skirt, less fat, and higher prices. Therefore, the development of parthenogenesis of all male seedlings of soft-shelled soft-shelled turtles can significantly improve economic benefits, and is of great significance to the development of the Chinese soft-shelled turtle breeding industry. [0004] The sex determination of soft-shelled turtles in normal development is female heterogamy, that is, the sex chromosomes of female soft-shelled turtles are ZW type, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02C12Q1/6888C12Q1/6851
CPCA01K67/02C12Q1/6888C12Q1/6851C12Q2600/166
Inventor 张君赵阳史新坤赵林斌
Owner ANHUI AGRICULTURAL UNIVERSITY
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