Primer and reagent kit for detecting serum exosome RP11-439M11-1, and application of primer or reagent kit to diagnosis and treatment of gastric cancer liver metastasis

A technology of exosomes and kits, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Inactive Publication Date: 2020-10-02
ZHENJIANG NO 1 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that CD44v6, OPN, YB-1, MAGE-A10 and other molecules may be used as potential markers of gastric cancer liver metastasis, but further verification is still needed

Method used

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  • Primer and reagent kit for detecting serum exosome RP11-439M11-1, and application of primer or reagent kit to diagnosis and treatment of gastric cancer liver metastasis
  • Primer and reagent kit for detecting serum exosome RP11-439M11-1, and application of primer or reagent kit to diagnosis and treatment of gastric cancer liver metastasis
  • Primer and reagent kit for detecting serum exosome RP11-439M11-1, and application of primer or reagent kit to diagnosis and treatment of gastric cancer liver metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Detection of RP11-439M11-1 in serum exosomes

[0036] 1. Obtain the serum sample to be tested:

[0037] Serum samples from 120 cases of gastric cancer and 120 normal persons.

[0038] The samples are the serum samples of the patients with gastric cancer and normal people with coagulant added.

[0039] 2. Extract exosomes from serum samples to be tested and verify

[0040] Extract exosomes from gastric serum according to the instructions of the exosome extraction kit, and the steps are as follows: (1) Whole blood is centrifuged at 3000×g for 15 min (centrifugal radius 10 cm) to remove cells or cell debris. (2) Put the upper liquid into a centrifuge tube, add 63 μl ExoQuick reagent to 250 μl serum, put it at 4°C for 30 minutes; (3) Centrifuge the mixture at 1500×g (centrifugal radius 10cm) for 30 minutes at 4°C; ( 4) Aspirate all the supernatant, centrifuge at 1500×g (centrifugal radius 10cm) for 5 min at 4°C, and aspirate the entire supernatant; (5) Dissolve...

Embodiment 2

[0071] Example 2 Clinical significance of serum exosome RP11-439M11-1

[0072] The correlation between serum exosomal RP11-439M11-1 expression and clinical parameters such as gender, age, tumor diameter, clinical stage, degree of tissue differentiation, lymph node metastasis, and liver metastasis of gastric cancer was further analyzed. See Table 4 and Table 5. It was found that the expression level of serum exosomal RP11-439M11-1 was significantly correlated with the depth of invasion, lymph node metastasis, liver metastasis, and clinical stage (P values ​​were 0.006, 0.028, 0.001, and 0.001, respectively). There was no significant correlation with gender, age, tumor location, tumor diameter, tissue differentiation, Helicobacter pylori infection and CEA level. The results of multivariate analysis suggested that the high expression of serum exosomal RP11-439M11-1 was one of the independent prognostic factors for gastric cancer liver metastasis.

[0073] Table 4 Clinical signif...

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Abstract

The invention relates to a primer, reagent kit and detection method for detecting a serum exosome RP11-439M11-1, and an application of the primer or the reagent kit to diagnosis and treatment of gastric cancer liver metastasis. The reagent kit comprises a RNA extraction reagent of a serum lncRNA sample, a primer, a genomic DNA removed reaction system, a RT reaction system, and a qPCR operation reaction system. The invention discloses the expression situation of lncRNA to gastric cancer patients and normal human serum exosomes, and fluorescent quantitation PCR is used for detecting the RP11-439M11-1. The serum exosome RP11-439M11-1 can be used for early diagnosis of gastric cancer liver metastasis, judging the disease progress and the prognosis situation, and can be used for evaluating thetreatment effect on the gastric cancer liver metastasis.

Description

technical field [0001] The invention relates to a primer, a kit of serum exosome RP11-439M11-1 and its application in the diagnosis and treatment of gastric cancer liver metastasis, belonging to the technical field of molecular diagnosis. Background technique [0002] Gastric cancer is a serious threat to human health and is one of the five major cancers in the world. The World Health Organization statistics report shows that in 2012, there were about 951,000 new cases of gastric cancer worldwide, and about 723,000 deaths. Gastric cancer rates are higher in East Asia (particularly China, Korea, Mongolia, and Japan), Central and Eastern Europe, South America, and most of Africa. China is the country with the highest incidence and mortality rate of gastric cancer in the world. According to data from the National Cancer Registry Center, there were about 679,000 new cases of gastric cancer in China in 2015, and about 498,000 deaths, ranking second among malignant tumors in my c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6886C12Q1/6851C12Q2600/178C12Q2600/118C12Q2600/158
Inventor 范钰何莲郎亚昆邹晨
Owner ZHENJIANG NO 1 PEOPLES HOSPITAL
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