Immunoglobulins capable of characterizing curative effect on ITP and application of immunoglobulins in preparing regent for detecting ITP
A technology of immunoglobulin and myosin, applied in the field of biomarkers, can solve the problems of cumbersome operation, time-consuming and laborious, etc., and achieve the effect of avoiding complicated and diagnostic procedures
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Embodiment 1
[0020] The inventors collected serum samples from normal control group, ITP patients before treatment and ITP patients' effective treatment group. As shown in the following table:
[0021] Table 1. Gender and age of ITP patients
[0022]
[0023] Note: There is no statistical difference between the two groups in gender and age, p>0.05;
[0024] First, patients must meet the following criteria for inclusion:
[0025] 1. Patients with chronic immune thrombocytopenia were clearly diagnosed by bone puncture;
[0026] 2. Exclude thrombocytopenia caused by other secondary factors;
[0027] 3. Platelet count > 10*10^9 / L; no obvious bleeding tendency;
[0028] 4. Patients without hepatic and renal insufficiency;
[0029] 5. Age > 18 years old.
[0030] Secondly, the criteria for treating this group of patients as effective include:
[0031] 1. Thrombocytopenia returned to normal or increased by 30*10^9 / L compared with before treatment;
[0032] 2. The platelet count did not...
Embodiment 2
[0039] On the basis of the above examples, this example: Protein data analysis identified that the content of two proteins, MRIgHV and IGHGγ3-like in the variable region of the myosin-reactive immunoglobulin heavy chain, was abnormally expressed in the ITP patient group, which was higher than that in the normal control group. Significantly down-regulated, but can be restored to similar expression levels in the control group after effective treatment. The two proteins were significantly different in the control group, the ITP patient group and the effective treatment group, such as figure 2 shown. The Gene Ontology enrichment analysis of the two proteins showed that the two proteins were mainly involved in endopeptidase activity, immune effector process, immune response, complement activation, protein activation cascade and reporter gene-mediated endocytosis. The results confirmed that these two proteins are highly correlated with the immune response, making them relevant mar...
Embodiment 3
[0041]On the basis of the above examples, this example: protein data analysis found that, along with the changes in the contents of MRIgHV and IGHGγ3-like proteins in each group of samples, there were corresponding changes in other proteins related to immune-related metabolic pathways, such as uniprot login The numbers are: MRIgHV protein family of Q9UL94, Q9UL75, Q9UL85, Q9UL82, Q9UL78, etc. For example, the heavy chain variable region protein of myosin responsive protein, the light chain variable region protein and other subunit proteins that make up the myosin responsive protein all have the same trend of change. Therefore, it can be guessed that these subunits together constitute the Immunoglobulin may be involved in the immune process of ITP. The details are as follows:
[0042] Table 3. Normal people, effective patients before treatment VS effective patients after treatment related protein expression
[0043]
[0044] Note: Only part of the data is shown.
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