Method for sampling and culturing neural stem cells

Abstract

The invention discloses a method for sampling and culturing neural stem cells. The method comprises the following steps: a, selecting qualified induced abortion embryonic tissues; b, separating embryotissues under a sterile condition: separating the embryo tissues, and finding and separating a neural plate or a neural tube formed by an ectoderm; c, obtaining a single-cell suspension conforming tothe inoculation concentration: taking out the ependymal membrane layer tissue under a sterile condition, cleaning the ependymal membrane layer tissue with a buffer solution, and separating the neuraltube epithelial cells by using a mechanical separation or enzymatic digestion separation method, inoculating the neural tube epithelial cells, at the density of 1*10<5> piece / ml, into a DMEM / F12 serum-free culture medium containing mitogenic inducers; d, carrying out subculture; and e, observing the cell growth condition via an inverted microscope. The method is innovative in the aspect of material taking tissues, the convenience of raw material taking is increased, original cells with higher activity are more effectively selected for culture, the culture success rate of the neural stem cellsis increased, and reliable guarantee is provided for clinical and basic research of various neural stem cells.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for obtaining and culturing neural stem cells. Background technique [0002] Stem cell research is a hotspot in current life science research, and neural stem cells are one of the most studied and most used types of stem cells. Since the conclusion that neural tissue cannot be regenerated was broken, neural stem cells have entered the field of vision of the majority of scientific researchers, and many clinicians have also made a lot of attempts. Some related theories are gradually becoming known to everyone: Many studies in the mid-1990s showed that human newborn neurons and glial cells come from neural stem cells, and most of them are concentrated in the olfactory bulb, the subventricular zone of the lateral ventricle, the hippocampus, and the spinal cord. They can differentiate into neurons and keratinocytes in vivo, and can generate neurospheres in vitro. Many propertie...

AI Technical Summary

Problems solved by technology

Of course, stem cells from other sources such as bone marrow, umbilical cord, amniotic fluid, etc. are also induced to differentiate into nerve cells across the germ layer, but the experimental operation is complicated

Now there are fewer and fewer qualified induced fetuses that can be directly used, and even scientific researchers have to spend a lot of time and energy to solve this problem, and patients have to spend most of their hospitalization time in waiting during the entire treatment process.

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