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Soluble microneedle patch and preparation method thereof

A soluble, microneedle patch technology, applied in the field of biomedical materials, can solve the problems of difficult storage of preparations, and achieve the effects of easy separation and culture, avoiding difficult storage problems, and convenient use

Inactive Publication Date: 2020-11-03
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a soluble microneedle patch and its preparation method, the purpose of which is to embed the culture supernatant extract of human mesenchymal stem cells in the microneedle, which is safe and , high efficiency, easy to use, and simple production process, thereby solving the technical problem of storage difficulties in the application of stem cells to the preparations containing living cells in the treatment of psoriasis

Method used

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  • Soluble microneedle patch and preparation method thereof
  • Soluble microneedle patch and preparation method thereof
  • Soluble microneedle patch and preparation method thereof

Examples

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Embodiment 1

[0045] This embodiment provides a method for preparing a dissolvable microneedle patch, including:

[0046] 1. Prepare the microneedle array mold: mix polydimethylsiloxane (PDMS, Sylgard184) and curing agent evenly according to the mass ratio of 10:1, and then pour the mixture solution on the surface of the microneedle template; then, vacuum Remove the air bubbles in the mixture, and the vacuum degree is -0.1~-0.05MPa; then heat and solidify the mixture at 70~80°C for 2~4h; after cooling, the solidified polydimethylsiloxane and the microneedle template Separated to obtain the PDMS microneedle array mold. Among them, see figure 1 and figure 2 , the microneedle body is in the shape of a cone, the height of the microneedle body is 2000 μm, the diameter of the bottom circle of the needle body 201 is 500 μm, and the center distance between the tips of two adjacent needle bodies 201 is 1000 μm, the microneedle The number is 30×30.

[0047] 2. Prepare human mesenchymal stem cell...

Embodiment 2

[0053] This embodiment provides a method for preparing a dissolvable microneedle patch, including:

[0054] 1. Prepare the microneedle array mold: mix polydimethylsiloxane (PDMS, Sylgard184) and curing agent evenly according to the mass ratio of 10:1, and then pour the mixture solution on the surface of the microneedle template; then, vacuum Remove the air bubbles in the mixture, and the vacuum degree is -0.1~-0.05MPa; then heat and solidify the mixture at 70~80°C for 2~4h; after cooling, the solidified polydimethylsiloxane and the microneedle template Separated to obtain the PDMS microneedle array mold. Among them, see image 3 , the microneedle body is in the shape of a regular triangular pyramid, the height of the microneedle body is 50 μm, the diameter of the circumscribed circle of the bottom surface of the needle body 201 is 50 μm, and the center distance between the tips of two adjacent needle bodies 201 is 100 μm, The number of microneedles is 30×30.

[0055] 2. Pre...

Embodiment 3

[0060] This example provides a method for preparing a soluble microneedle patch. The difference between this example and Example 1 is that step 2 in this example is to use cell growth medium to cultivate bone marrow-derived human mesenchyme in an in vitro sterile environment. stem cells.

[0061] In this example, during the culture process of human mesenchymal stem cells, human mesenchymal stem cells will secrete various active cytokines into the supernatant of the cell culture medium. The polymer solution is mixed, and finally a solid dissolvable microneedle patch is obtained. The solid-state soluble microneedle patch can be stored at room temperature in a dry environment, which effectively avoids the problem of difficult storage of various stem cells in the application process in the prior art.

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Abstract

The invention discloses a soluble microneedle patch and a preparation method thereof. The soluble microneedle patch comprises a sheet-shaped substrate and a soluble polymer microneedle formed on the sheet-shaped substrate, wherein a human mesenchymal stem cell culture supernatant extract is embedded in the soluble polymer microneedle. The method comprises the following steps: culturing human mesenchymal stem cells in a cell culture medium, performing filtering to remove the human mesenchymal stem cells to obtain a supernatant, and performing degerming to obtain a human mesenchymal stem cell culture supernatant extract solution; mixing the human mesenchymal stem cell culture supernatant extract solution with a soluble polymer solution to obtain a mixed solution, injecting the mixed solutioninto a microneedle mold, enabling the whole microneedle mold to be filled with the mixed solution through vacuum treatment, performing drying, and performing stripping on the microneedle mold to obtain the soluble microneedle patch. The soluble microneedle patch is safe, efficient, convenient to use, simple in production process and capable of being stored at normal temperature.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and more specifically relates to a dissolvable microneedle patch and a preparation method thereof. Background technique [0002] Microneedles (MNs) are needle-like micron-scale fine array structures. Microneedles can assist drugs to enter the skin through the stratum corneum, and their length is not enough to touch the subcutaneous pain nerves. Advantage. Common microneedle materials mainly include silicon, metal and polymer materials. Among them, inorganic materials are prone to safety problems due to their poor biocompatibility, high brittleness, and easy breakage in the skin. Soluble polymer materials can completely dissolve or degrade in the skin, are easy to use, and have good biocompatibility, so they are widely used in the preparation of microneedles. [0003] Psoriasis is a very common chronic inflammatory skin disease. The typical symptom is erythema on the skin covered w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K47/34A61K47/36A61K35/28A61P17/06A61M37/00
CPCA61K9/0021A61K35/28A61K47/34A61K47/36A61M37/0015A61M2037/0023A61M2037/0053A61P17/06
Inventor 杜虹瑶王华陶娟朱锦涛张连斌
Owner HUAZHONG UNIV OF SCI & TECH
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