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Reagent kit, measurement kit, and measurement method

A technology of kits and surfactants, applied in measuring devices, biological tests, instruments, etc., can solve the problem of high hydrophobicity

Pending Publication Date: 2020-11-03
FUJIFILM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, it is known that SAA may be due to its high hydrophobicity, and most of it is usually associated with high-density lipoprotein (high-density lipoprotein: HDL) in blood

Method used

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  • Reagent kit, measurement kit, and measurement method
  • Reagent kit, measurement kit, and measurement method
  • Reagent kit, measurement kit, and measurement method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0171](1) Production of latex particles with an average particle diameter of 150nm

[0172] Suspend 30 g (288 mmol) of styrene (manufactured by FUJIFILM Wako Pure Chemical Corporation) and 3 g (42 mmol) of acrylic acid (manufactured by FUJIFILM Wako Pure Chemical Corporation) in 440 mL of ultrapure water, raise the temperature to 95° C., and add potassium persulfate (KPS) ( FUJIFILM Wako Pure Chemical Corporation (manufactured by FUJIFILM Wako Pure Chemical Corporation) 1 g was dissolved in an aqueous solution of 10 mL of ultrapure water, and stirred at 95° C. and 250 rpm for 6 hours. Thereafter, centrifugation was performed three times at 10,000 rpm for 6 hours to obtain latex particles. Finally, the obtained latex particles were dispersed in ultrapure water again. Pure water was added so that the solid content concentration became 1 mass %, and the diluted liquid was prepared. The average particle diameter of the latex particles was 150 nm when calculated as a median parti...

Embodiment 2

[0197] Prepare and use the fluorescent latex particles modified with the anti-SAA antibody used in Example 1, and further add the latex particles modified with the anti-CRP antibody that is not fluorescently labeled in a mass ratio of 5 times Except for drying the particles, in the same manner as in Example 1, the rate of increase in fluorescence signal value was determined for each of test samples No. 1 and No. 2.

Embodiment 3～9

[0199] In Example 1, instead of surfactant D316, the surfactants and concentrations shown in Table 1 were changed, and in the same manner as in Example 1, for test samples No.1 and No.2, respectively, the The increasing speed of the fluorescence signal value.

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Abstract

The present invention addresses the problem of providing a reagent kit, measurement kit, and measurement method for immunologically measuring serum amyloid A with high accuracy and high sensitivity, without using an alcohol designated as a hazardous material in the fire protection code. The present invention provides a reagent kit that is for measuring serum amyloid A and that contains: first particles labelled and modified with a first binding substance capable of specifically binding to serum amyloid A; and at least one nonionic surfactant having a molecular weight of 1000 or less.

Description

technical field [0001] The invention relates to a test kit for measuring serum amyloid A, a test kit for measuring serum amyloid A and a method for measuring serum amyloid A in biological samples. Background technique [0002] Conventionally, in bioassays and the like, fluorescence detection methods have been widely used as highly sensitive and easy measurement methods. This fluorescence detection method is a method of confirming the presence of the detection target substance by detecting fluorescence at the time of irradiating a sample thought to contain a detection target substance excited by light of a specific wavelength to fluoresce with excitation light of the above-mentioned specific wavelength. In addition, when the detection target substance is not a fluorescent substance, a substance that specifically binds to the detection target substance labeled with a fluorescent dye is brought into contact with the sample, and then fluorescence is detected in the same manner a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/543G01N33/545
CPCG01N33/545G01N33/543C07K14/4711G01N33/6893G01N2800/7095G01N33/92G01N2333/775G01N33/5306G01N33/533G01N33/54346G01N33/68G01N2333/47
Inventor 大内亮中村和浩
Owner FUJIFILM CORP
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