Super-hydrophobic three-channel synchronous detection upconversion fluorescence probe detection test piece and preparation method thereof

A simultaneous detection and fluorescent probe technology, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, and material analysis through optical means, to achieve the effects of sensitive sensing detection ability and good self-cleaning ability

Active Publication Date: 2020-11-10
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the technical problem that a single up-conversion fluorescent probe cannot perform simultaneous detection of multiple analytes and single-droplet circulation detection, and provides a super-hydrophobic three-way synchronous detection up-conversion fluorescent probe detection test piece and its preparation method

Method used

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  • Super-hydrophobic three-channel synchronous detection upconversion fluorescence probe detection test piece and preparation method thereof
  • Super-hydrophobic three-channel synchronous detection upconversion fluorescence probe detection test piece and preparation method thereof
  • Super-hydrophobic three-channel synchronous detection upconversion fluorescence probe detection test piece and preparation method thereof

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specific Embodiment approach 1

[0053] Specific embodiment 1: The superhydrophobic three-way synchronous detection up-conversion fluorescent probe detection test piece of this embodiment consists of a substrate 1, an up-conversion nanocrystalline layer 2 with a multilayer core-shell structure and superhydrophobic SiO 2 The covering layer 3 consists of a multilayer core-shell structure up-conversion nanocrystalline layer 2 deposited on the substrate 1, superhydrophobic SiO 2 A capping layer 3 is deposited on the up-conversion nanocrystalline layer 2 of the multilayer core-shell structure.

[0054] The up-conversion nanocrystal layer 2 of the multi-layer core-shell structure is composed of the up-conversion nanocrystal of the multi-layer core-shell structure, and the up-conversion nanocrystal of the multi-layer core-shell structure is coated on the surface of the crystal core 2-1 from the inside to the outside Formed by the first functional layer 2-2, the first isolation layer 2-3, the second functional layer ...

specific Embodiment approach 2

[0061] Specific embodiment two: the preparation method of the superhydrophobic three-way synchronous detection up-conversion fluorescent probe detection test piece described in specific embodiment one is carried out according to the following steps:

[0062] 1. Preparation of upconversion nanocrystals with multilayer core-shell structure:

[0063] (1) Preparation of crystal nucleus NaYF 4 :(18%~22%)Yb 3+ / (0.5%~1.5%)Tm 3+ ;

[0064] (2) Prepare the first functional layer NaYF on the surface of the crystal nucleus 4 :(10%~30%)Yb 3+ / (10%~30%)Nd 3+ layer;

[0065] (3) Prepare the first isolation layer NaLuF on the surface of the first functional layer 4 layer;

[0066] (4) Prepare the second functional layer NaYF on the surface of the first isolation layer 4 :(18%~22%)Yb 3+ / (1%~3%)Er 3+ layer;

[0067] (5) Prepare the second isolation layer NaLuF on the surface of the second functional layer 4 layer;

[0068] (6) Prepare the third functional layer NaErF on the sur...

specific Embodiment approach 3

[0081] Specific embodiment three: the difference between this embodiment and specific embodiment two is the crystal nucleus NaYF in step one (1) 4 :(18%~22%)Yb 3+ / (0.5%~1.5%)Tm 3+ The preparation method is as follows:

[0082] a: Press NaYF 4 :(18%~22%)Yb 3+ / (0.5%~1.5%)Tm 3+ Stoichiometric ratio Weigh the total amount of 0.8 ~ 1.2mmol of YCl 3 ·6H 2 O, YbCl 3·6H 2 O, TmCl 3 ·6H 2 O is put into 250mL three-neck flask;

[0083] b: Take 6-9mL of oleic acid and 12-18mL of octadecene into the flask of step a, connect the reaction device and pass in argon gas, heat to 130-150°C to completely dissolve the added rare earth raw materials in the organic solution, Keep for 25-35 minutes;

[0084] c: Cool down to 40-50°C, add 0.10-0.12g NaOH and 0.13-0.16g NH 4 For the methanol solution of F, keep stirring for 20-30 minutes to form crystal nuclei, then raise the temperature to 70-90°C and keep for 20-30 minutes to remove methanol;

[0085] d: After the methanol is removed,...

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Abstract

The invention discloses a super-hydrophobic three-channel synchronous detection up-conversion fluorescence probe detection test piece and a preparation method thereof, and relates to a dye detection test piece and a preparation method thereof. The invention aims to solve the technical problem that a single up-conversion fluorescence probe cannot simultaneously detect various to-be-detected objectsand circularly detect a single liquid drop. The detection test piece is composed of a substrate, an up-conversion nanocrystalline layer of a multi-layer core-shell structure and a super-hydrophobic SiO2 covering layer, wherein nanocrystalline of the up-conversion nanocrystalline layer of the multi-layer core-shell structure is formed by sequentially depositing a first functional layer, a first isolation layer, a second functional layer, a second isolation layer, a third functional layer and a third isolation layer on the surface of a crystal nucleus. The preparation method comprises the following steps: 1, preparing an up-conversion nanocrystal with the multi-layer core-shell structure; 2, preparing silane modified hydrophobic SiO2 particles; and 3, preparing a super-hydrophobic up-conversion film. The detection test piece provided by the invention can realize synchronous detection of three mixed dyes, has a detection limit of about 0.1 [mu]g / mL, and can be used in the field of dye detection.

Description

technical field [0001] The invention relates to the simultaneous detection of multiple dyes and the repeated use detection technology of superhydrophobic up-conversion fluorescent probes. Background technique [0002] Organic dyes are widely used in the fields of textile, dyeing, printing, cosmetics, food coloring, and papermaking. Up to now, more than 100,000 kinds of organic dyes have been developed, with an annual production of about 7×10 5 tons of organic dyes. About 12% of the dyes are discharged into the environment as waste. Wastewater containing these toxic and carcinogenic dye molecules has caused serious environmental pollution problems. Therefore, the accurate detection of various dyes in wastewater has become an important research topic. [0003] Fluorescent sensors are widely used in the fields of chemical and biological analysis due to their high detection sensitivity, low background interference and high selectivity. Among many fluorescent materials, up-co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/85G01N21/64
CPCC09K11/7773C09K11/025C09K11/02G01N21/6428
Inventor 郝树伟陈童尚云飞杨春晖
Owner HARBIN INST OF TECH
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