A kind of serial culture kit for regenerating Hooke ginger flower plant and its application

A technology of Hooke ginger flowers and plants, applied in the field of plant tissue culture, can solve problems such as differences in the concentration ratio of plant growth regulators, and achieve the effects of simplified disinfection operation steps, fast induction speed and high regeneration efficiency.

Active Publication Date: 2022-07-01
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in these technical schemes, the types of plant growth regulators selected and their concentration ratios are very different among different plants.

Method used

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  • A kind of serial culture kit for regenerating Hooke ginger flower plant and its application
  • A kind of serial culture kit for regenerating Hooke ginger flower plant and its application
  • A kind of serial culture kit for regenerating Hooke ginger flower plant and its application

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Experimental program
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Effect test

Embodiment 1

[0037] The present invention is an embodiment of the serial culture kit for regenerating Huke ginger flower plants and its application. The present embodiment is used for the series culture kit for regenerating Huke ginger flower plants, including seed germination medium, callus tissue Induction medium, callus proliferation medium and callus redifferentiation medium.

[0038] In the seed germination medium, the concentration of sucrose is 28 g / L, and the concentration of agar is 7 g / L; in the callus induction medium, the concentration of kinetin is 0.1 mg / L, 2,4-dichloro The concentration of phenoxyacetic acid is 0.5mg / L, the concentration of sucrose is 28g / L, and the concentration of agar is 7g / L; in the callus proliferation medium, the concentration of kinetin is 0.1mg / L, 2,4 - The concentration of dichlorophenoxyacetic acid is 0.3 mg / L, the concentration of sucrose is 28 g / L, and the concentration of agar is 7 g / L; in the callus redifferentiation medium, the concentration o...

Embodiment 2

[0047] The present invention is an embodiment of the serial culture kit for regenerating Huke ginger flower plants and its application. The present embodiment is used for the series culture kit for regenerating Huke ginger flower plants, including seed germination medium, callus tissue Induction medium, callus proliferation medium and callus redifferentiation medium.

[0048] In the seed germination medium, the concentration of sucrose is 29 g / L, and the concentration of agar is 7.5 g / L; in the callus induction medium, the concentration of kinetin is 0.3 mg / L, 2,4-di The concentration of chlorophenoxyacetic acid was 0.8 mg / L, the concentration of sucrose was 29 g / L, and the concentration of agar was 7.5 g / L; in the callus proliferation medium, the concentration of kinetin was 0.15 mg / L, 2 , The concentration of 4-dichlorophenoxyacetic acid was 0.4 mg / L, the concentration of sucrose was 29 g / L, and the concentration of agar was 7.5 g / L; The concentration was 1.5 mg / L, the conc...

Embodiment 3

[0051] The present invention is an embodiment of the serial culture kit for regenerating Huke ginger flower plants and its application. The present embodiment is used for the series culture kit for regenerating Huke ginger flower plants, including seed germination medium, callus tissue Induction medium, callus proliferation medium and callus redifferentiation medium.

[0052] In the seed germination medium, the concentration of sucrose is 30 g / L, and the concentration of agar is 8 g / L; in the callus induction medium, the concentration of kinetin is 0.5 mg / L, 2,4-dichloro The concentration of phenoxyacetic acid is 1.0mg / L, the concentration of sucrose is 30g / L, and the concentration of agar is 8g / L; in the callus proliferation medium, the concentration of kinetin is 0.2mg / L, 2,4 - the concentration of dichlorophenoxyacetic acid is 0.5mg / L, the concentration of sucrose is 30g / L, and the concentration of agar is 8g / L; in the callus redifferentiation medium, the concentration of 6...

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Abstract

The invention relates to a series of culture kits for regenerating Huke ginger flower plants and applications thereof, belonging to the field of plant tissue culture. The serial culture kit for regenerating Huke ginger flower plants of the present invention includes: a seed germination medium, a callus induction medium, a callus proliferation medium and a callus redifferentiation medium. The Huke ginger flower callus regeneration system of the invention has simple operation steps, low explant contamination rate, high callus induction speed, high regeneration efficiency, no need for seedling strengthening and rooting steps, and one-step seedling formation.

Description

technical field [0001] The invention relates to a series of culture kits for regenerating Huke ginger flower plants and applications thereof, belonging to the field of plant tissue culture. Background technique [0002] Compared with other plants of the same genus, Hooke ginger flower has a short plant, broad leaves, light yellow flower color and black aril, which has important research and development and utilization value. The plant's callus regeneration system is the basic technology platform for further genetic manipulation and breeding improvement of the plant. At present, in the genus Ginger, the types of established callus regeneration systems include White Ginger and Red Ginger. These two species belong to the type with thick rhizomes and thin lateral roots, and are distributed at medium and low altitudes, while Hooke ginger flower has small rhizomes, thick lateral roots, and is distributed at high altitudes, and the species are quite different. In the selection of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李永泉胡秀张媚谭嘉川
Owner ZHONGKAI UNIV OF AGRI & ENG
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