The invention also discloses application of TMTT as drug for resisting lepidoptera pests from invading crops

A Lepidoptera and drug technology, applied in the fields of application, pest control, and pest repellent, can solve the problems of TMTT avoidance and direct poisoning of insects that have not been reported in the literature, so as to inhibit growth and development and reduce pupation rate effect

Active Publication Date: 2020-12-29
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Terpenes (3E,7E)-4,8,12-trimethyltridecan-1,3,7,11-tetraene, (3E,7E)-4,8,12-trimethyltridecan-1,3 ,7,11-tetraene, molecular formula C 16 h 26 , the relative molecular weight is 218.2035, referred to as TMTT, is a natural plant secondary metabolite, this compound can be used as an inducer to induce the natural enemies of pests to kill insects, but there is no literature report that TMTT has the effect of avoiding and directly poisoning insects

Method used

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  • The invention also discloses application of TMTT as drug for resisting lepidoptera pests from invading crops
  • The invention also discloses application of TMTT as drug for resisting lepidoptera pests from invading crops
  • The invention also discloses application of TMTT as drug for resisting lepidoptera pests from invading crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Plutella xylostella is to TMTT odor selection experiment

[0020] (1) Dissolve TMTT in paraffin, and dilute to a sample concentration of 75ng / μl for use.

[0021] (2) The 2nd instar diamondback moth was starved for 0.5h.

[0022] (3) Use a hole puncher to drill feed with uniform size, apply the sample prepared in the first step evenly on the surface of the feed and put it into the two ends of the insect odor selective device, the feed coated with paraffin is set as the control group, and the feed coated with TMTT is set For the experimental group, they were placed 8cm inward from the ports at both ends, connected to an air sampler, and pumped for 10 minutes to form a flowing air flow in the channels at both ends.

[0023] (4) Put 15 3rd instar early stage diamondback moth larvae of the same growth into the middle interface of the insect odor selection device, and continue to pump air.

[0024] (5) Count the number of diamondback moth larvae crawling to b...

Embodiment 2

[0026] Example 2: The toxic effect of TMTT on the cruciferous crop pest Plutella xylostella.

[0027] (1) Dissolve TMTT in paraffin, and dilute to a sample concentration of 75ng / μl for use.

[0028] (2) The 2-day-old diamondback moth was starved for 0.5h.

[0029] (3) The experiment set up a control group and an experimental group, and each group set up 6 parallel repetitions. Use a puncher to drill feeds of uniform size, add three feeds to each plate, and add 7.27 μl of paraffin oil (control group) or 7.27 μl TMTT samples (experimental group) to each feed, that is, each feed in the experimental group contains 545.25 ng of TMTT, put 15 Plutella xylostella larvae into each group, and put them into a constant temperature incubator at 25°C for cultivation.

[0030] (4) The mortality rate, body weight and later pupation rate of diamondback moth were counted every day.

[0031] The experimental results showed that with the increase of feeding TMTT time, compared with the control...

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Abstract

The invention relates to an application of TMTT as a drug for resisting lepidoptera pests invading crops, and belongs to the technical field of pesticide preparation. TMTT is one of aroma components released by plants and belongs to terpene compounds. Existing researches show that the TMTT can attract pollination insects and attract natural enemies of phytophagous insects. The invention firstly discovers that TMTT has a repelling effect and a poisoning effect on lepidoptera pests, namely plutella xylostella. The substance belongs to a natural secondary metabolite and does not pollute soil anda water source, so that development of a novel efficient pollution-free natural insecticide by taking the substance as a research object has important significance on prevention and control of crop pests.

Description

technical field [0001] The invention belongs to the technical field of pesticide manufacture, and in particular relates to the application of TMTT as a medicine against lepidopteran pests invading crops. Background technique [0002] Plutella xylostella, also known as caterpillar, is an important pest of cruciferous vegetables such as cabbage, rapeseed, cauliflower, and cabbage, and is also a pest widely distributed in the world. Because of its many generations, strong reproductive ability, wide host range, high level of drug resistance and typical insect evolution advantages, it brings great difficulties to the control work, so it is particularly important to develop new control technologies for diamondback moth. [0003] The existing Plutella xylostella control technologies mainly include agricultural control, physical control, mechanical control and biological control. At the same time, the scientific and comprehensive utilization of these four control technologies can im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01P7/04A01P17/00
CPCA01N27/00
Inventor 李培金陈红亿王传宏陶珍黄世界陈晨江桃山
Owner ANHUI AGRICULTURAL UNIVERSITY
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