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Application of Chemical Elicitors in Promoting Stem Growth and Development of Sandalwood Seedlings

A chemically induced, sandalwood technology, applied in the fields of application, botanical equipment and methods, horticulture, etc., can solve the problems of lack of young sandalwood research, no available information, etc.

Active Publication Date: 2022-06-21
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above studies have described the effects of some chemical elicitors on the formation of sandalwood heartwood, most of the research materials are sandalwood aged 6 years and above, and there is a lack of research on young sandalwood
In particular, the combination of anatomy, morphology and molecular biology to study the effects of foliar spraying of various chemical elicitors on the growth, wood formation and transcriptional regulation of essential oil synthesis of 1-year-old sandalwood has not been available in previous studies. information

Method used

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  • Application of Chemical Elicitors in Promoting Stem Growth and Development of Sandalwood Seedlings
  • Application of Chemical Elicitors in Promoting Stem Growth and Development of Sandalwood Seedlings
  • Application of Chemical Elicitors in Promoting Stem Growth and Development of Sandalwood Seedlings

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Experimental program
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Embodiment 1

[0023] 1. Materials and methods

[0024] 1. Plant growth conditions and material selection

[0025] First, the plump seeds of sandalwood (Santalum album L.) (after removing the aril) were soaked in 2 mM gibberellin solution for 12 hours, and then the surface of the seeds was disinfected with 3% sodium hypochlorite solution for 5 minutes, and after rinsing with distilled water for 3 times , sown in sterile sand in nursery (ambient temperature: 27-32°C) pots. After the seeds have germinated and the seedlings have grown at least 4 leaves (it takes about 1 month to 1.5 months), the 10cm tall seedlings are moved into 14cm x 11cm plastic filled with a mixture of loess and humus (5:1, v / v) Pots, placed in a shady shed. After 1 month, the seedlings were cultured in association with the host Artemisia annua. When the seedlings grew to more than 30 cm, the seedlings and their host Artemisia annua were jointly transferred to a 25 cm × 30 cm cloth bag filled with a mixture of loess and...

Embodiment 2

[0051] 1. Materials and methods

[0052] 1. Test materials and drugs

[0053] The test sandalwood was planted in 2010. The early hosts were mainly sandalwood, such as Mirle, Jiuli, and Neem. The row spacing was about 2m × 2m. The experimental plot was a flat terrain with good drainage.

[0054] In October 2015, 44 sandalwood plants with similar growth and diameter at breast height of 6-7cm and yet to form heartwood were randomly selected from the experimental site for testing.

[0055] The tested drugs are: 6-Benzylaminopurine (BA) (~99%), Shanghai Ika Biotechnology Co., Ltd.; Ethephon (ETH) (≥85%), Beijing Soleibao Technology Ltd; 30% H 2 O 2 (Analytical grade), Sinopharm Chemical Reagent Co., Ltd.; Calcium Chloride (>99%), Beijing Combes Technology Co., Ltd.; Methyl jasmonate (MeJ) (95%), Aladdin Reagent (Shanghai) Ltd. Each drug has two concentrations of 2mM and 4mM, and the dosage is about 400ml / time, 4 times a year. The reagent preparation method is shown in Table 1...

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Abstract

The invention discloses the application of chemical elicitors in promoting the growth and development of sandalwood seedling stems. This study explores the BA, ETH, MeJA, H 2 o 2 and CaCl 2 Effects on the growth and development of 1-year-old sandalwood and the expression of key genes for essential oil synthesis. These inducers have positive effects on promoting the growth and development, wood formation and expression of santalene synthase gene SaSSY of sandalwood seedlings, especially 1-year-old sandalwood stems; BA, ETH, MeJA and H 2 o 2 It has a positive effect in inducing the expression of the SaCYP736A167 gene (a key gene regulating santalol synthesis). The above research results provide a theoretical basis for the study of the formation of sandalwood wood and heartwood, and the molecular mechanism of santalenol biosynthesis, and also provide a basis for the use of chemical inducers (the best inducers BA and H 2 o 2 ) provide practical basis for scientific guidance of artificial cultivation of sandalwood.

Description

Technical field: [0001] The invention belongs to the field of sandalwood industry, and particularly relates to the application of chemical elicitors in promoting the growth and development of sandalwood seedling stems. Background technique: [0002] Sandalwood (Santalum album L.) is known for its hard, beautifully textured heartwood and compounds such as odoriferous, high-quality sandalol extracted from the heartwood, and has great commercial value. However, unlike other short-rotation timber-producing species, sandalwood grows slowly and has a long heartwood formation cycle. In natural forests in India, sandalwood grown for 10 to 13 years gradually forms heartwood, and sandalwood grown to 20 years old has the highest growth rate of heartwood. The high benefits of sandalwood products are the main reason for its over-exploitation, which seriously hinders the sustainable development of its productivity. Since 1980, the native resources of sandalwood have declined sharply in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 李媛马国华金峰程庆伟张新华
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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