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Application of chemical inducers in promoting growth and development of sandalwood seedling stems

A chemical induction, growth and development technology, applied in the fields of application, botany equipment and methods, horticulture, etc., can solve the problems of lack of young sandalwood research and unavailable information

Active Publication Date: 2021-01-08
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above studies have described the effects of some chemical elicitors on the formation of sandalwood heartwood, most of the research materials are sandalwood aged 6 years and above, and there is a lack of research on young sandalwood
In particular, the combination of anatomy, morphology and molecular biology to study the effects of foliar spraying of various chemical elicitors on the growth, wood formation and transcriptional regulation of essential oil synthesis of 1-year-old sandalwood has not been available in previous studies. information

Method used

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  • Application of chemical inducers in promoting growth and development of sandalwood seedling stems
  • Application of chemical inducers in promoting growth and development of sandalwood seedling stems
  • Application of chemical inducers in promoting growth and development of sandalwood seedling stems

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Experimental program
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Effect test

Embodiment 1

[0023] 1. Materials and methods

[0024] 1. Plant growth conditions and material selection

[0025] First, soak the plump seeds of sandalwood (Santalum album L.) (after removing the aril) in 2mM gibberellin solution for 12 hours, then disinfect the surface of the seeds with 3% sodium hypochlorite solution for 5 minutes, rinse with distilled water for 3 times , sow in sterile sand in pots in a nursery (ambient temperature: 27-32° C.). After the seeds germinate and the seedlings grow at least 4 leaves (it takes about 1 month to 1.5 months), move the 10cm high seedlings into a 14cm×11cm plastic container filled with a mixture of loess and humus (5:1, v / v). Pots, placed in a shady shed. After 1 month, the seedlings were cultured together with the host Artemisia annua. When the seedlings grew to more than 30 cm, the seedlings and their hosts, Artemisia annua, were transferred to a 25 cm × 30 cm cloth bag filled with a mixture of loess and humus (5:1, v / v) to continue culturing. ...

Embodiment 2

[0051] 1. Materials and methods

[0052] 1. Test materials and drugs

[0053] The sandalwood for testing was planted in 2010, and the early hosts were mainly sandalwood such as Miruihua, Jiulixiang, and Neem. The row spacing was about 2m×2m, and the test plot was a flat terrain with good drainage.

[0054] In October 2015, 44 sandalwood trees with similar growth, diameter at breast height of 6-7cm, and heartwood had not yet formed were randomly selected in the test site for testing.

[0055] The tested drugs are: 6-Benzylaminopurine (6-Benzylaminopurine, BA) (~99%), Shanghai Yika Biotechnology Co., Ltd.; Ethephon (ETH) (≥85%), Beijing Suolaibao Technology Limited; 30% H 2 o 2 (Analytical grade), Sinopharm Chemical Reagent Co., Ltd.; Calcium Chloride (>99%), Beijing Combis Technology Co., Ltd.; Methyl jasmonate (MeJ) (95%), Aladdin Reagent (Shanghai) Ltd. Each drug has two concentrations of 2mM and 4mM, and the dosage is about 400ml / time, 4 times a year. See Table 1 for r...

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Abstract

The invention discloses an application of chemical inducers in promoting growth and development of sandalwood seedling stems. The research discusses the influence of BA, ETH, MeJA, H2O2 and CaCl2 on the growth and development of one-year-old sandalwood and the expression of essential oil synthesis key genes. The inducers play a positive role in promoting growth and development of sandalwood seedlings, especially one-year-old sandalwood stems, wood formation and sandalene synthase gene SaSSY expression; and the BA, ETH, MeJA and H2O2 have positive effects in the aspect of inducing the expression of an SaCYP736A167 gene (regulating and controlling the synthesis key gene of santalol). The research result provides a theoretical basis for researching sandalwood wood and heartwood formation andresearching a sandalenol biosynthesis molecular mechanism, and also provides a practical basis for scientifically guiding sandalwood artificial cultivation by utilizing chemical inducers (the optimalinducers BA and H2O2).

Description

Technical field: [0001] The invention belongs to the field of sandalwood industry, and in particular relates to the application of chemical elicitors in promoting the growth and development of sandalwood seedling stems. Background technique: [0002] Sandalwood (Santalum album L.) is well-known for its hard and beautifully textured heartwood, its aromatic and high-quality sandalol and other compounds extracted from the heartwood, and has huge commercial value. However, unlike other short-rotation wood-producing species, sandalwood grows slowly and has a long heartwood formation cycle. In natural forests in India, sandalwood that has grown for 10 to 13 years gradually forms a heartwood, and sandalwood that has grown to 20 years has the highest heartwood development speed. The high profit of sandalwood products is the main reason for its over-exploitation, which seriously hinders the sustainable development of its productivity. Since 1980, the original resources of sandalwoo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 李媛马国华金峰程庆伟张新华
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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