Steroid compounds in podocarpus nagi leaves as well as extraction method and application of steroid compounds

A steroid compound and compound technology, applied in the field of medicine, can solve the problems of difficult cancer treatment and poor overall effect, and achieve the effect of rapid separation and identification and strong inhibitory activity

Active Publication Date: 2021-02-02
XIAMEN INST OF RARE EARTH MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most cancer patients are already in the middle to late stage when they are diagnosed with the disease, and the overall effect o

Method used

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  • Steroid compounds in podocarpus nagi leaves as well as extraction method and application of steroid compounds
  • Steroid compounds in podocarpus nagi leaves as well as extraction method and application of steroid compounds
  • Steroid compounds in podocarpus nagi leaves as well as extraction method and application of steroid compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Extraction, separation and structural identification of compounds A and B

[0042] The extraction and separation of compounds A and B is carried out according to the following process:

[0043] (A) Grind 20 kg of dried bamboo and cypress leaves (collected from Xiangshendai Village, Yangli, Fujian Province), put them into two 20L plastic buckets, add 10L of 70% alcohol / water solution, soak at room temperature for 1 month, filter and concentrate That is the extract. The above-mentioned soaking, filtering, and concentrated extraction were repeated three times for the two barrels of medicinal materials, and the obtained extracts were combined.

[0044] (B) Disperse 1 kg of extract obtained in step A in 1000mL water, and extract 5 times with sherwood oil, ethyl acetate, chloroform, n-butanol successively, each time sherwood oil, ethyl acetate, chloroform, n-butanol consumption 500ml. Concentrate the extracts of different polarities respectively to obtain extrac...

Embodiment 2

[0051] Embodiment 2: in vitro antitumor activity test

[0052] The preferred compound A was tested for its anti-tumor activity in vitro, and its inhibitory activity on colorectal cancer cell line and breast cancer cell line MCF-7 was mainly studied. The specific test process is described by taking the test process of the breast cancer MCF-7 cell line as an example:

[0053] 1. Test sample concentration preparation

[0054] Weigh 10 mg of compound A, add it to a 5 mL plastic centrifuge tube, and dilute to 2 mL with DMSO.

[0055] That is, the initial concentration of 5mg / mL was obtained. Then the initial concentration was diluted with DMSO to obtain 6 different concentration gradients of 5mg / mL, 2.5mg / mL, 1.25mg / mL, 0.625mg / mL, 0.3125mg / mL, and 0.15625mg / mL in sequence. Store in refrigerator at 4°C until use.

[0056] 2. Culture and inhibitory activity test of breast cancer cell line (MCF-7)

[0057] Place breast cancer cell line MCF-7 at 37°C, saturated humidity, containi...

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Abstract

The invention provides a method for extracting and separating compounds A and B. The method comprises the following steps: carrying out primary purification (such as column separation, preparation plate separation and the like) on an extracting solution of podocarpus nagi leaves, conducting recrystallizing, and selecting different crystals to obtain high-purity compounds A and B. According to themethod, two steroid compounds are successfully separated from the podocarpus nagi leaves, and the two steroid compounds have very strong inhibitory activity on various cancer cells (such as gastric cancer cell strains, colorectal cancer cell strains and breast cancer cell strains). In addition, the method for purifying the compound provided by the invention can be used for rapidly and accurately obtaining a high-purity target compound, and has important significance for rapid separation and identification of specific compounds in a plant extracting solution containing complex components.

Description

technical field [0001] The invention belongs to the technical field of medicine. In particular, it relates to a steroidal compound isolated from leaves of bamboo and cypress, an extraction method thereof and an application in treating tumor / cancer. Background technique [0002] Cancer has become one of the most important lethal diseases worldwide. Cancer can occur in various organs and tissues at any age. The main types of cancer that cause death are: lung cancer, stomach cancer, liver cancer, colon cancer and breast cancer. Although some small molecule anticancer drugs have been used clinically, some compounds are undergoing preclinical research. However, most cancer patients are already in the middle to late stage when they are diagnosed with the disease, and the overall effect of clinical treatment is poor, especially the continuous emergence of multi-drug resistant cancer cells, which makes cancer treatment difficult. Therefore, it is imminent to develop new anticance...

Claims

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Application Information

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IPC IPC(8): C07J9/00A61K31/575A61P35/00
CPCA61P35/00C07J9/00
Inventor 雍建平卢灿忠杨洋
Owner XIAMEN INST OF RARE EARTH MATERIALS
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