Method for constructing type II diabetes model by combining high-fat feeding with streptozotocin induction

Abstract

The invention relates to a method for constructing a type II diabetes model by combining high-fat feeding with streptozotocin induction. According to the method, the high-fat and high-sugar diet is given by simulating the morbidity process of human type II diabetes, so that wide insulin resistance of mice is caused, but the blood sugar is not obviously increased. STZ is a powerful alkylating agentand can cause DNA alkylation, the DNA chain is broken, so that glycosylation of poly adenosinediphosphate ribose is activated, NO is released, the content of nicotinamide adenine in cells is rapidlyreduced, pancreatic beta cells are damaged or necrotic, insulin secretion is reduced, at the moment, most mice have abnormal glucose tolerance, and animal hyperglycemia is induced. The method has themain beneficial effects that the diabetes model of the female ICR mouse is successfully established through the research, and the pathological structure and biochemical detection change trend of the mouse are basically consistent with those of type II diabetes of human beings.

Description

[0001] (1) Technical field [0002] The invention relates to a method for constructing an ICR mouse type II diabetes model by high-fat feeding combined with streptozotocin induction. [0003] (2) Background technology [0004] Diabetes mellitus (DM) is a type of metabolic disease that causes glucose and lipid metabolism disorders in the body due to absolute or relative insufficiency of insulin. Its incidence is increasing year by year worldwide. The third largest health killer after tumors. To replicate the experimental animal model of diabetes close to the human body is a key link in diabetes research. At present, the methods for preparing animal models of diabetes mainly include chemical agent induction, spontaneous screening, transgenic and gene knockout, pancreatectomy, special diet induction or co-induction models, etc. At home and abroad, male rats are generally used to prepare diabetes models, and the research on the preparation of rat diabetes models is relatively in-...

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Problems solved by technology

At home and abroad, male rats are generally used to prepare diabetes models, and the research on the preparation of rat diabetes models is relatively in-depth, but there are few studies on mouse diabetes models, especially female mouse diabetes models.

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