Targeting vector for Stap2 gene point mutation knock-in mode mouse and construction method

A technology for targeting vectors and construction methods, which can be applied to other methods of inserting foreign genetic materials, biochemical equipment and methods, genetic engineering, etc., and can solve problems such as the unexplained function of Stap2 protein and the inability to study the function.

Pending Publication Date: 2021-03-12
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the previous studies on it were mainly in cell lines, and there were few related research reports. The function of Tyr250 of Stap2 protein in vivo has not been elucidated, and

Method used

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  • Targeting vector for Stap2 gene point mutation knock-in mode mouse and construction method
  • Targeting vector for Stap2 gene point mutation knock-in mode mouse and construction method
  • Targeting vector for Stap2 gene point mutation knock-in mode mouse and construction method

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[0061] In order to make the above objects, features, and advantages of the present invention, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to fully understand the present invention. However, the present invention can be implemented in many other fails there are many otherwise described herein, and those skilled in the art may do similar improvements without departing from the connotation of the present invention, and thus the present invention is not limited by the specific embodiments disclosed below.

[0062] All techniques and scientific terms used herein are identical to those skilled in the art, unless otherwise defined. The terms used herein in the specification of the present invention are intended to describe specific embodiments, and is not intended to limit the invention.

[0063] First design the target scheme, see figure 1...

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Abstract

The invention discloses a targeting vector for a Stap2 gene point mutation knock-in mode mouse and a construction method. The construction method comprises the following steps: constructing a targeting vector, designing sgRNA and preparing microinjection RNA of the sgRNA, preparing an F0-generation mouse by utilizing Cas9, the microinjection RNA and the targeting vector, carrying out genotype identification, preparing an F1-generation mouse, carrying out genotype identification, carrying out Sothern blot identification and the like. The Stap2 gene belongs to an important linker protein familyand can perform a signal transduction function by sensing an extracellular cell signal. The targeting vector of the Stap2 protein Tyr250 deleted mouse and the construction method of the Stap2 proteinTyr250 deleted mouse are proposed for the first time, a specific model can be provided for research of Stap2 protein Tyr site deletion, the blank of the research direction is filled, and the effect ofthe site can be explored more deeply.

Description

technical field [0001] The invention belongs to the technical field of mouse model construction, and in particular relates to a targeting vector and a construction method of a Stap2 gene point mutation knock-in model mouse. Background technique [0002] Activation of intracellular signaling is regulated by the transduction of many signaling molecules through extracellular ligands such as cytokines, hormones and growth factors. In the signaling pathway, adapter proteins play an important role in signal transduction. Although adapter proteins do not have any catalytic function, their unique domains can help them transduce signals to specific targets. [0003] The Stap2 gene (full name is signal transducing adapter family member 2, also known as: AW049765, STA, STAP-2) is on the reverse strand of chromosome 17, with a total length of about 8.53kb. Gene ID: 106766. The Stap2 gene, which was first obtained from a human mammary gland cDNA library in 2000 by yeast two-hybrid tec...

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Application Information

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IPC IPC(8): C12N15/85C12N15/90A01K67/027
CPCC12N15/8509C12N15/907A01K67/0276A01K2217/075A01K2227/105A01K2267/03Y02A50/30
Inventor 王建荣袁娜魏雯
Owner SUZHOU UNIV
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