Vector system for inducing apoptosis

A carrier system and cell-inducing technology, which is applied in the field of biomedicine, can solve the problems of complex recombination process and time-consuming, and achieve the effect of simple preparation process, intuitive experimental results, and short onset period

Active Publication Date: 2021-03-30
GUIZHOU MEDICAL UNIV
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Problems solved by technology

At present, tumor gene drugs, targeted therapy, and specific cell elimination strategies based on the Cre-LoxP recombinase system are popular, but the recombination process is complicated and time-consuming, and generally requires sever

Method used

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  • Vector system for inducing apoptosis
  • Vector system for inducing apoptosis
  • Vector system for inducing apoptosis

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Embodiment

[0024] A vector system for inducing cell apoptosis, including recombinant vector 1 and recombinant vector 2, recombinant vector 1 is Plasmid CMV-FKBP-tdTomato, recombinant vector 2 is Plasmid EF1α-FRBCaspase8-2A-BSD, recombinant vector 1 and recombinant vector 2 The sequences are shown in SEQ ID NO: 3 and SEQ ID NO: 4; the recombinant vector one is formed by connecting the FKBP gene fragment and the backbone plasmid tdTomato-N1, and the FKBP gene fragment is a gene sequence fragment with F36V and L106P mutation sites. The encoded and synthesized protein has poor stability, but has stronger binding ability with other proteins. The sequence of the FKBP gene fragment is shown in SEQ ID NO: 1; the recombinant vector 2 is formed by connecting the FRB Caspase8-2A-BSD fragment with the backbone plasmid lenti Cas9-Blast The fragment of FRB Caspase8-2A-BSD is FRB with three point mutations (FRB*), which is designed to bind rapamycin analogs. The sequence of FRB Caspase8-2A-BSD fragment ...

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Abstract

The invention relates to the field of biomedicine, and discloses a vector system for inducing apoptosis, wherein the vector system comprises Plasmid CMV-FKBP-tdTomato and Plasmid EF1[alpha]-FRBCaspase8-2A-BSD. The invention further discloses a preparation method of the vector system. The invention also discloses an application of the vector system. The vector system is simple in preparation process, can be locally imported and expressed at a specific part, is direct in system action and lesion organism method, is short in onset period, can shorten the research time, can independently control the marking and clearing processes, and is more intuitive in experiment result; therefore, the vector system disclosed by the invention can be widely applied to mechanism research and treatment strategy exploration of diseases, such as in-vivo lesion cell distribution research of the diseases and occurrence and treatment relationship research of lesion cells and the diseases.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a carrier system for inducing cell apoptosis. Background technique [0002] In biomedical research, the occurrence of various diseases such as tumors, gene-related diseases, and aging-related diseases is caused by special pathological changes in certain cells in the body. For this cell-specific or intracellular phenotype The targeted removal of specific abnormal cells is of great significance for the study of the pathological mechanism of diseases and the exploration of disease treatment strategies. At present, tumor gene drugs, targeted therapy, and specific cell elimination strategies based on the Cre-LoxP recombinase system are popular, but the recombination process is complicated and time-consuming, and generally requires several generations of reproduction and genetic modification in animals. In the screening process, for the exploration of disease treatment strategies, there are...

Claims

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Application Information

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IPC IPC(8): C12N15/867C12N15/66C12N15/65C12N15/57C12N15/12
CPCC12N15/86C12N15/65C12N9/6472C07K14/4738C07K14/4702C12Y304/22061C12N2740/15043C12N2800/107
Inventor 齐晓岚王晓玲何志旭刘健胡晓霞
Owner GUIZHOU MEDICAL UNIV
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