39results about How to "The experimental results are intuitive" patented technology

The invention belongs to a quantitative detection method of an enzyme, and in particular relates to an HPRT (hypoxanthine phosphoribosyl transferase) body gene mutation detection method based on surface plasmon resonance. The HPRT body gene mutation detection method based on surface plasmon resonance is characterized by comprising the following steps: (1) preparation of a wild HPRT enzyme-sensitive SPR (surface plasmon resonance) gold film; (2) SPR detection of a wild HPRT standard substance; and (3) sample preparation and the SPR detection: after collected mammal cells are repeatedly washed by a hanks liquid, directly adding a histiocyte lysis solution; vibrating at the temperature of 4DEG C to cause the sample to fully crack; carrying out low-temperature centrifugation on the cracked product; collecting a supernate containing proteins; replacing the lysis solution component in the supernate by PBS (phosphate buffered saline), and storing in a refrigerator at the temperature of 4 DEGC; and carrying out SPR detection. The method has high detection sensitivity, and the content of wild HPRT of ngmL-1 order of magnitude can be detected.

The invention discloses a schering bridge based insulating gas liquefaction temperature testing device and method. The schering bridge based insulating gas liquefaction temperature testing device comprises a thermostat, a constant volume bottle, an electrode couple, a gas distributing device, a liquid phase detection device and a data monitoring and processing unit; the constant volume bottle is arranged in the thermostat, a thermometer and a pressure gauge are arranged in the constant volume bottle; the electrode couple is arranged on the wall of the constant volume bottle, a discharge end ofthe electrode couple is located in the constant volume bottle, the two ends of the electrode couple located outside the constant volume bottle are connected with a high voltage power supply to form aloop; the liquid phase detection device comprises a schering bridge; a detection end of the schering bridge is arranged in the constant volume bottle; the gas distributing device is communicated withthe constant volume bottle; and the thermometer, the pressure gauge, the thermostat, the gas distributing device and the liquid phase detection device are respectively communicated with the data monitoring and processing unit through a signal line. The testing device solves the problem of proximity existing in the calculated value of the ideal gas equation, and the liquefaction temperature of theinsulating gas is tested on site and verified.

The invention provides a projectile motion experimental instrument and an experimental instrument system. The projectile motion experimental instrument comprises a magnetic ball body, a throwing device, a display panel, a ball receiving groove and a base. The display panel comprises a display screen, a sensing circuit and a controller. The sensing circuit comprises a plurality of magnetic sensors and a scanning circuit board. The plurality of magnetic sensors are arranged on the scanning circuit board at a preset interval. After the magnetic ball body is thrown out of the throwing device, the magnetic ball body conducts the parabolic movement at the front part of the display panel. During the parabolic movement, the magnetic ball body triggers the conduction of magnetic sensors at corresponding positions of the sensing circuit. The scanning circuit board sends the position signals of conducted magnetic sensors to the controller. The controller is used for sending a display signal to the display screen according to the position signals of conducted magnetic sensors, so that the display screen can display the motion track of the magnetic ball body in real time. According to the technical scheme of the invention, the projectile motion experimental operation is simple and efficient. Furthermore, the teaching effect of the projectile motion experiment is improved.

The invention discloses a test device used for testing insulating gas liquefaction temperature and a test method used for testing insulating gas liquefaction temperature. The test device comprises a constant temperature box, a constant volume bottle, a gas distributing device, a liquid phase detection device and a data monitoring and processing unit. The temperature range of the constant temperature box is adjustable. The constant volume bottle is arranged in the constant temperature box. The constant volume bottle is internally provided with a temperature meter and a pressure meter. The liquid phase detection device comprises an optical fiber scene processing unit and a schering bridge. The liquid phase detection device is connected and communicated with the constant volume bottle. The detection end of the schering bridge is arranged in the constant volume bottle. The gas distributing device is communicated with the constant volume bottle. The temperature meter, the pressure meter, the constant temperature box, the gas distributing device and the liquid phase detection device are connected with the data monitoring and processing unit through a signal line. According to the test device, the approximation problem existing on the basis of the ideal gas equation calculation value can be overcome and the insulating gas liquefaction temperature can be tested and verified on site; and two test methods of high operability are integrated to perform mutual verification of the test accuracy of the liquefaction temperature.

The invention discloses a method for analyzing the diversity of biological enzyme genes in compost, which comprises the following steps: first extracting the total DNA of microorganisms in compost, and the extraction process further includes three steps: decomposing of samples, rough extraction of DNA and purification of DNA. Steps; then use the PCR amplification program to amplify the specific biological enzyme gene; then run denaturing gradient gel electrophoresis in 1×TAE buffer at a constant temperature of 60°C and a constant voltage of 100V for 12 to 14 hours; finally, denaturing gradient gel electrophoresis Gel electrophoresis patterns were analyzed to determine the diversity of specific biological enzyme genes in the compost. The analysis method of the present invention has the advantages of high throughput, low cost, simplicity and intuition, etc., so as to better characterize the microbial population with degradation function in the compost.

The invention discloses a pulmonary alveoli surfactant function experiment device and method. The device comprises a communication tube, a first balloon and a second balloon, and the first balloon iscommunicated with the second balloon through the communication tube. The device is simple in structure, the experiment method is easy to operate, the functions of water molecules and surfactants in the pulmonary alveoli under a microscopic condition are displayed through a macroscopic phenomenon, teachers are helped to explain and analyze knowledge points, and then students conveniently understandthe knowledge points.

The invention discloses an experimental method for researching influence on railway ballast splash in the train driving process. A determination method of initial detection sites comprises the following steps: placing three lines of simulation particles on two sides of a train railway in the width direction and in the center of the railway, wherein each line of simulation particles comprise a plurality of simulation particles placed along the length direction of the train railway; recording the position of each simulation particle after each movement when the train drives over the train railway, and after every time the train drives over the train railway, putting each simulation particles at original positions respectively, and connecting the adjacent positions after movement of the simulation particles to form the activity range of the simulation particles; in each line of simulation particles, marking the average value of the superposed areas of every two adjacent simulation particles in the activity range as A, marking the average value of the areas of the simulation particles in the activity range as B, and adjusting the distance of the adjacent simulation particles until thevalue of A / B is 0 to 30%, namely obtaining the initial detection sites of the simulation particles.

The invention relates to a microphysics simulation experiment apparatus and method for oil displacement by water; the apparatus is characterized by comprising an oil microscopic observation system, a liquid level laser scanning system, a computer terminal, a first specimen and a second specimen; the liquid level laser scanning system comprises a platform, a support frame, a motor, a screw, a shifting table, a laser sensor, a waste liquid recycler, a support and a hydraulic pump; the support frame and the motor are arranged on two sides of the top of the platform in opposite manner; the second specimen is arranged at the top of the support frame, and the waste liquid recycler is arranged at the top of one end of the support frame; a shifting table capable of moving parallelly is arranged on a screw of the motor, and the laser sensor is arranged on the shifting table; a support is arranged at the bottom of the platform, and the hydraulic pump that is liftable is arranged between the bottom of the platform and the support; the laser sensor is connected with a computer terminal and is used for laser-scanning the second specimen and transmitting scanning data to the computer terminal. The microphysics simulation experiment apparatus and method are widely applicable to microphysics simulation experimental researches in the petroleum field.