The invention relates to an instrument used for environmental science research, and specifically relates to a microorganism BOD measuring system for continuous oxygen supply capable of providing accurate temperature control. The system has the working principle that under the conditions of constant temperature and normal pressure, biological degradation is carried out on microorganisms in a reaction bottle, O2 is consumed for generating CO2, after CO2 is absorbed by an absorbent, the pressure inside the reaction bottle is reduced, pressure difference is generated, and a circuit inside a U-shaped pipe is communicated. The current inside the U-shaped pipe is too small, and the starting of a direct current power supply module cannot be triggered. Therefore, an electrical signal is amplified to control the starting of the direct current power supply assembly through a micro signal amplifier, a CuSO4 solution inside an electrolysis bottle is electrolyzed to generate O2, and simultaneously the quantity of electric charge of electrolysis is recorded by a coulombmeter; and when O2 is supplemented into the reaction bottle, the pressure difference inside the pressure gage with the U-shaped pipe disappears, the circuit is disconnected, the direct current power supply assembly is turned off, the electrolysis bottle stops electrolysis, and the coulombmeter stops recording. Due to the fact that the oxygen consumption is in direct proportion to the quantity of electric charge of electrolysis, the electric quantity consumed by the electrolysis bottle is accurately monitored by the coulombmeter, and the BOD value is converted through the electric quantity.