Method for analyzing diversity of biological enzyme genes in compost

Abstract

The invention discloses a method for analyzing diversity of biological enzyme genes in a compost, comprising the following steps of: at first, extracting microbial total DNA in the compost, wherein, the extraction procedure also comprises three steps of dehumification of a sample, crude extraction of the DNA and purification of the DNA; afterwards, utilizing a PCR amplification program to amplify specific biological enzyme genes; then implementing denaturing gel gradient electrophoresis for 12-14 hours in 1XTAE buffer solution at the constant temperature of 60 DEG C and under the constant voltage of 100V; and finally, analyzing a denaturing gel gradient electrophoresis map to determine the diversity of the specific biological enzyme genes in the compost. The inventive analysis method has the advantages of high flux, low cost, great simplicity and being visual, in order to better represent microbial population having a function degradation in the compost.

Description

technical field [0001] The invention relates to a method for gene diversity, in particular to an analysis method for gene diversity in the total DNA of composting microorganisms. Background technique [0002] Composting is a system in which microorganisms are used to decompose biodegradable solid waste to achieve its final treatment. Therefore, a full understanding of the microbiological principles can provide a basis for improving the treatment process and improving treatment efficiency. The traditional methods for microbial community research on compost are mainly microbial culture and pure species isolation techniques. Because the vast majority of microorganisms in the environment cannot be studied by culture methods, and there are many kinds of microorganisms in compost, which are often in a state of dynamic change, and culture research cannot reflect the dynamic changes of microbial populations in the system. Therefore, such research methods cannot meet the needs of com...

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the separation and diversity of functional genes in the environment should be combined with the method of constructing a clone library, but this method is not suitable for the research on the separation and diversity of various functional genes in the composting process. The reasons are: construction A clone library must pick dozens or even hundreds of clones at a time, and composting is a dynamic process that is constantly changing. Therefore, if you want to isolate multiple functional genes in the composting process and conduct subsequent diversity research It is necessary to construct multiple clone libraries, which not only greatly increases the workload, but also increases the experimental cost to a certain extent

Images