Analytical method for bioenzyme gene diversity in compost

Abstract

The invention discloses a method for analyzing the diversity of biological enzyme genes in compost, which comprises the following steps: first extracting the total DNA of microorganisms in compost, and the extraction process further includes three steps: decomposing of samples, rough extraction of DNA and purification of DNA. Steps; then use the PCR amplification program to amplify the specific biological enzyme gene; then run denaturing gradient gel electrophoresis in 1×TAE buffer at a constant temperature of 60°C and a constant voltage of 100V for 12 to 14 hours; finally, denaturing gradient gel electrophoresis Gel electrophoresis patterns were analyzed to determine the diversity of specific biological enzyme genes in the compost. The analysis method of the present invention has the advantages of high throughput, low cost, simplicity and intuition, etc., so as to better characterize the microbial population with degradation function in the compost.

Description

technical field [0001] The invention relates to a method for gene diversity, in particular to an analysis method for gene diversity in the total DNA of composting microorganisms. Background technique [0002] Composting is a system in which microorganisms are used to decompose biodegradable solid waste to achieve its final treatment. Therefore, a full understanding of the microbiological principles can provide a basis for improving the treatment process and improving treatment efficiency. The traditional methods for microbial community research on compost are mainly microbial culture and pure species isolation techniques. Because the vast majority of microorganisms in the environment cannot be studied by culture methods, and there are many kinds of microorganisms in compost, which are often in a state of dynamic change, and culture research cannot reflect the dynamic changes of microbial populations in the system. Therefore, such research methods cannot meet the needs of com...

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the separation and diversity of functional genes in the environment should be combined with the method of constructing a clone library, but this method is not suitable for the research on the separation and diversity of various functional genes in the composting process. The reasons are: construction A clone library must pick dozens or even hundreds of clones at a time, and composting is a dynamic process that is constantly changing. Therefore, if you want to isolate multiple functional genes in the composting process and conduct subsequent diversity research It is necessary to construct multiple clone libraries, which not only greatly increases the workload, but also increases the experimental cost to a certain extent

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