Preparation method of milk powder standard substance containing aflatoxin AFTM1

Abstract

The invention relates to a preparation method of a milk powder matrix standard substance containing aflatoxin AFTM1. The preparation method is mainly used for calibrating a measuring instrument and evaluating the accuracy of a measuring method. The invention belongs to the technical field of method detection. The method comprises the following steps: obtaining raw milk polluted by AFTM1; removing physical impurities, performing cooling to 3-5 DEG C, and injecting the product into a milk storage tank for storage; performing preheating, and keeping the temperature at 40-50 DEG C without exceeding 60 DEG C; preforming homogenizing, and keeping the homogenizing pressure at 5-20 Mpa; performing sterilizing, controlling the temperature at 80-85 DEG C, and performing concentrating until the solid content is about 40%-50%; performing spray drying, the pressure range of a high-pressure pump being 5-20 Mpa; keeping the temperature of milk powder at an outlet of a fluidized bed at 25-30 DEG C; and performing cooling to room temperature, performing sieving, uniformly performing mixing, performing sealing and sub-packaging. The milk raw material polluted by the aflatoxin AFTM1 is obtained by simulating feeding, and the milk powder sample polluted by the AFTM1 in actual detection is completely simulated by the procedures of impurity removal, homogenization, spray drying and the like, so that the accuracy and the reliability of a detection result are improved.

Description

technical field [0001] The present invention relates to a kind of aflatoxin-containing AFTM 1 The preparation method of milk powder matrix standard substance is a kind of measurement standard, which is mainly used for calibrating measuring instruments and evaluating the accuracy of measurement methods. Background technique [0002] Aflatoxin M 1 (Aflatoxin M 1 , AFM 1 ), formula C 17 h 12 o 7 ; Molecular weight 328; CAS No. 6795-23-9; Melting point: 299°C; Rectangular flake shape, colorless crystal. Soluble in chloroform, acetonitrile, methanol and other organic solvents and water. AFTM1 has a stable structure and high temperature resistance, and it cannot be destroyed by pasteurization. [0003] Aflatoxin AFM 1 Produced directly by some Aspergillus flavus and Aspergillus parasitica, but with other toxins such as AFTB 1 、AFTB 2 、AFTG 1 、AFTG 2 ), the ratio is quite low. AFTM in Milk and Dairy Products 1 , usually by AFB due to ingestion by dairy animals 1 Aft...

AI Technical Summary

Problems solved by technology

Due to the complex composition of milk, the way of adding standard products, AFTM 1 The combination with the matrix is ​​inconsistent with the real test sample, which cannot represent the real presence of the target in food, which affects the extraction efficiency of the target and leads to deviations in the test results

However, the screening of pollutants is difficult to meet the demand for the quality of matrix standard substances.

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