Method for establishing efficient regeneration system of sapium sebiferum stem segment

A technology for high-efficiency regeneration and establishment of methods, applied in plant regeneration, horticultural methods, botany equipment and methods, etc., can solve the problems of low tissue culture efficiency, dead leaves and seedlings, easy browning, etc., and achieve short cultivation period and high survival rate High, easy-to-operate effect

Active Publication Date: 2021-04-16
JIANGSU ACAD OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also a small number of reports on the tissue culture of Chinese tallow tree, but there are serious problems such as easy browning, fallen leaves and dead seedlings, and at the same time, there are problems of low tissue culture efficiency and long cycle

Method used

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  • Method for establishing efficient regeneration system of sapium sebiferum stem segment
  • Method for establishing efficient regeneration system of sapium sebiferum stem segment
  • Method for establishing efficient regeneration system of sapium sebiferum stem segment

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Experimental program
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Effect test

Embodiment 1

[0030] 1) Sterile material acquisition

[0031] Cut the branches of Chinese tallow tree that grow robustly and are free of diseases and insect pests, and cut the branches into stem segments with a length of about 1.5 cm, a total of 600, and ensure that each stem segment has a full bud body, and the growth position of the bud is located at The upper 1 / 3 of the stem segment. Put the cut stems into a glass beaker, and rinse them under tap water for 30 min. Soak in 800 times carbendazim solution for 30 minutes, soak in 20% 84 disinfectant solution for 30 seconds, rinse the stems with distilled water 5 times, and put them into the ultra-clean workbench. Soak in 75% alcohol for 30 s, soak in 15% sodium hypochlorite solution (3 drops of Tween 80 per liter) for 6 min, rinse with sterile water for 5 times, and dry the surface of the stem with sterile filter paper;

[0032] 2) Stem segments induce bud germination

[0033] The obtained stem segment explants were evenly inoculated into...

Embodiment 2

[0039] 1) Sterile material acquisition

[0040] Cut the branches of Chinese tallow tree that grow robustly and are free from diseases and insect pests, and cut the branches into stem segments with a length of about 2.0 cm, a total of 600, to ensure that there is a full bud on each stem segment, and the growth position of the bud is located on the stem. 1 / 3 of the upper end of the segment. Put the cut stems into a glass beaker, and wash them under tap water for 40 min. Soak in 900-fold carbendazim solution for 30 min, soak in 20% 84 disinfectant for 30 s, rinse the stems with distilled water 5 times, put them in an ultra-clean workbench, soak them in 75% alcohol for 30 s, put them in 15 % sodium hypochlorite solution (3 drops of Tween 80 per liter) were soaked for 8 min, washed with sterile water for 5 times, and the moisture on the surface of the stem segment was blotted dry with sterile filter paper;

[0041] 2) Stem segments induce bud germination

[0042] The obtained st...

Embodiment 3

[0048] 1) Sterile material acquisition

[0049] Cut the branches of Chinese tallow tree that grow robustly and are free from diseases and insect pests, and cut the branches into stem segments with a length of about 3.0 cm, a total of 600, to ensure that there is a full bud on each stem segment, and the growth position of the bud is located on the stem. 1 / 3 of the upper end of the segment. Put the cut stems into a glass beaker, and wash them under tap water for 100 min. Soak in 1000 times carbendazim solution for 30 min, soak in 20% 84 disinfectant for 30 s, rinse the stem section with distilled water 5 times, and put it in the ultra-clean workbench. Soak in 75% alcohol for 30 s, soak in 15% sodium hypochlorite solution (3 drops of Tween 80 per liter) for 10 min, rinse with sterile water for 5 times, and dry the surface of the stem with sterile filter paper;

[0050] 2) Stem segments induce bud germination

[0051] The obtained stem segment explants were inoculated into thre...

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Abstract

The invention relates to a method for establishing an efficient regeneration system of a sapium sebiferum stem segment. The method comprises the following steps: 1, preparing a sterile material, namely a sterile sapium sebiferum stem segment; 2, conducting germination of stem segment induced buds; 3, conducting bud induction rooting; and 4, seedling hardening and transplanting. The buds with the stem length of 1.0-1.5 cm are cut, 1 / 2 MS serves as a basic culture medium, IBA with the concentration of 0.5 mg / L and NAA with the concentration of 0.05 mg / L are added, and good rooting can be achieved within 15-20 d. The sapium sebiferum seedlings are subjected to two stages of gradual seedling strengthening processes in a tissue culture chamber and are transplanted to a mixed matrix composed of turfy soil, perlite and vermiculite (3:2:1), soil nutrition, water content and permeability of the sapium sebiferum seedlings are guaranteed, and the transplanting survival rate is greatly increased. A sapium sebiferum tissue culture regeneration system is established by the method, operation is simple and convenient, the pollution rate is low, the survival rate is high, and the culture period is short.

Description

technical field [0001] The invention provides a method for establishing a regeneration system of Chinese tallow tree, which can effectively reduce the cultivation cost of Chinese tallow tree. Background technique [0002] Sapium ( Sapium sebiserum ), also known as wax tree and wax tree, is a deciduous tree of the Euphorbiaceae Sapium genus. It is light-loving, drought-tolerant, and can tolerate short-term water accumulation. It can also grow in saline-alkali land with a salinity rate below 0.3%. Chinese tallow tree is widely distributed in my country, and mostly cultivated in the Yangtze River and Pearl River basins. It is a unique economic tree species in my country. Chinese tallow tree is one of the four major woody oil plants in my country, and it is also a rare ornamental tree species for landscaping. Its crown is neat, its leaves are beautiful, and the leaves are red and beautiful after autumn. It can be planted alone or clustered on lawns, lakesides and pools. In land...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01H4/00
Inventor隋德宗王保松宁坤马艳
OwnerJIANGSU ACAD OF FORESTRY