Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of pb in the preparation of drugs for preventing or treating pilo-induced epilepsy

A technology for epilepsy and epileptic mice, which is applied in drug combinations, pharmaceutical formulas, medical preparations containing active ingredients, etc., to achieve the effects of reducing seizure levels, slowing down weight loss, and prolonging the incubation period

Active Publication Date: 2021-11-16
THE AFFILIATED HOSPITAL OF SHANDONG UNIV OF TCM
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the inventors have found that various bioactive ingredients extracted from existing animals and plants have certain preventive and preventive effects on EP.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of pb in the preparation of drugs for preventing or treating pilo-induced epilepsy
  • Application of pb in the preparation of drugs for preventing or treating pilo-induced epilepsy
  • Application of pb in the preparation of drugs for preventing or treating pilo-induced epilepsy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Experimental part of embodiment 1

[0047] 1. Establishment of EP model

[0048] After C57BL / 6J mice were bred adaptively for 1 week, the mice were injected with atropine sulfate (1 mg / kg) intraperitoneally to antagonize the peripheral cholinergic response, and PILO (280 mg / kg) was injected intraperitoneally 15 minutes later. 60 minutes after the appearance of EP symptoms, The reaction was terminated by intraperitoneal injection of diazepam (10 mg / kg). According to the Racine grading evaluation, the model preparation was considered successful if the seizure level reached 4-5. The specific indicators of Racine classification are as follows: grade 1: facial twitching; grade 2: nodding; grade 3: unilateral forelimb clonic seizure; grade 4: standing up, bilateral forelimb clonic seizure; land.

[0049] 2. Grouping and administration of animals

[0050] Fifteen C57BL / 6J mice were randomly selected as the blank group, and the mice with successful model preparation were ra...

Embodiment 2

[0065] Embodiment 2 result discussion

[0066] 1. Effect of PB on body weight and survival rate of EP mice

[0067] On the first day of the experiment, there was no significant difference in the body weight of the mice in each group (P≥0.05). On the 28th day, compared with the blank group, the body weight of the mice in the model group was significantly reduced (P≤0.05), suggesting that the long-term EP attack had a certain inhibitory effect on the growth of the body weight of the mice; compared with the model group, the mice in the low-dose group There was no significant change in body weight (P ≥ 0.05), and the middle-dose group and high-dose group could significantly increase the body weight of EP mice (P ≤ 0.05), indicating that PB administration can inhibit the decline in body weight of EP mice; Compared with the dose group, there was no significant difference in the body weight change of the mice in the middle dose group (P ≥ 0.05), and there was a significant differenc...

Embodiment 3

[0098] Based on the previous research on Gastrodia elata with alcohol, the present invention first explores the preliminary intervention effect of PB components on the epileptic behavior of EP mice induced by PILO. "Shen Nong's Materia Medica" records that Gastrodia elata "taste is pungent, warm..., long-term use can benefit energy, grow yin, fat and healthy, lighten the body and increase the age." This study shows that PB administration can inhibit the weight loss trend of EP mice, and has A certain dose-dependent, while improving the survival rate of EP mice, prolonging the onset latency of EP, shortening the duration of the state and reducing the level of seizures, PB has a certain effect on improving the occurrence of epileptic behavior in EP mice. This study will focus on PB The development of innovative anti-epileptic drugs lays a research foundation.

[0099] EP attack or EP continuous state will damage the cognitive function of the brain. Improving brain function damag...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of pharmaceutical preparations, and in particular relates to the application of barisenoside B (PB) in the preparation of drugs for preventing or treating epilepsy induced by pilocarpine (PILO). For the first time, it was found that barisenoside B can significantly prolong the latency period of pilocarpine-induced epilepsy, shorten the duration of status epilepticus, reduce the grade of epileptic seizures, and slow down the weight loss caused by epilepsy, so it has a good effect on PILO-induced epilepsy Preventive and therapeutic effects. It has important clinical application value in pilocarpine-induced epilepsy, and at the same time opens up a new drug use for the active ingredient of barisonoside B.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to the application of barisenoside B (PB) in the preparation of drugs for preventing or treating epilepsy induced by pilocarpine (PILO). Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Epilepsy (epilepsy, EP) is a chronic, recurrent, and progressive chronic disease caused by excessive and abnormal discharge of brain nerve cells. The main clinical manifestation is sudden onset accompanied by transient brain dysfunction. Nervous system diseases that focus on prevention and treatment. [0004] At present, the clinical treatment of EP is mainly to take antiepil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7034A61P25/08
Inventor 王信李莉刘天易师伟马传江杨培民
Owner THE AFFILIATED HOSPITAL OF SHANDONG UNIV OF TCM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com