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Preparation and application of zilpaterol hapten, complete antigen and monoclonal antibody

A monoclonal antibody and complete antigen technology, applied in the field of immunoassay, can solve problems such as labor consumption, lengthy sample processing procedures, and extensive use restrictions

Inactive Publication Date: 2021-04-20
XINXIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods can achieve accurate quantitative and qualitative detection of ZIL in different samples, the instruments are expensive, time-consuming, and labor-intensive, and require dedicated laboratories and operators, as well as lengthy and strict sample processing procedures. The widespread use of this detection method has been greatly limited

Method used

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  • Preparation and application of zilpaterol hapten, complete antigen and monoclonal antibody
  • Preparation and application of zilpaterol hapten, complete antigen and monoclonal antibody
  • Preparation and application of zilpaterol hapten, complete antigen and monoclonal antibody

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Embodiment

[0057] Preferred embodiments of the present invention provide the preparation and application of zilpaterol hapten, complete antigen and monoclonal antibody, specifically as follows:

[0058] The solution system used includes:

[0059] 0.01M, pH=7.4 Phosphate Buffer Saline (PBS): 40.24g KH2PO, 1.44g Na2HPO4, 8g NaCl, 0.2g KCl, add about 800mL of deionized water and stir to dissolve, then add concentrated hydrochloric acid to adjust the pH to 7.4, and finally Dilute to 1L and store at room temperature.

[0060] 1mol / L NaOH solution: Weigh 4g NaOH and dissolve it in 100mL deionized water, and store in the dark at 4°C.

[0061] 0.1mol / L MES solution: Weigh 0.168g MES and dilute to 10mL deionized water, and store at 4°C in the dark.

[0062] 0.1mol / L KOH solution: Weigh 0.28g of KOH and dissolve it in deionized water, then transfer it to a 50mL volumetric flask, and dilute to the mark with deionized water.

[0063] 1.5molL, pH=8.8 Tris-HCL separating gel buffer: 18.15g Tris was...

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Abstract

The invention discloses preparation and application of a zilpaterol hapten, a zilpaterol complete antigen and a zilpaterol monoclonal antibody, and comprises the zilpaterol hapten, the zilpaterol complete antigen and the zilpaterol monoclonal antibody and the preparation method thereof, and further comprises the application of the zilpaterol monoclonal antibody in prepation of a kit / collaurum chromatography test strip for detecting Zilpaterol residues. The prepared complete antigen has good immunogenicity and is used for preparing an efficient and stable monoclonal antibody, the high specificity of the monoclonal antibody and the high sensitivity of the method are organically combined, rapid antigen detection is achieved, and the complete antigen is suitable for detecting a large number of samples in a short time and can be used for detecting zilpaterol residues in the samples.

Description

technical field [0001] The invention belongs to the technical field of immune detection, in particular to the preparation and application of a zilpaterol hapten, a complete antigen and a monoclonal antibody. Background technique [0002] Zipaterol (Zilpaterol, ZIL) is a β 2 - Adrenoceptors (β 2 -adrenergic receptors (β2AR) agonists, like ractopamine (RAC) and clenbuterol (CLB), belong to the "clenbuterol" category, and its main function is to promote protein synthesis and muscle growth, and reduce fat tissue precipitation. When fed to animals as a feed additive, it can significantly increase the muscle / fat ratio of the animal, significantly increase the lean meat rate and feed conversion rate of the raised animal carcass, significantly improve the quality of the meat and reduce the cost of animal breeding. [0003] When the body takes excessive ZIL, it will cause vasodilation and rapid heartbeat, and it may remain in the tissues and organs of the body, such as liver, kidn...

Claims

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Application Information

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IPC IPC(8): C07D487/06C07K14/765C07K14/77C07K16/44G01N33/53G01N33/543G01N33/577G01N33/58
Inventor 王选年郭东光陈明艳岳锋李文明李梦阳孙国鹏潘鹏涛李鹏朱艳平
Owner XINXIANG UNIV
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