Method for breeding lotus triploid by 2n pollen hybridization
A triploid, lotus technology, applied in the fields of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of heavy workload, undisclosed processing procedures, and high costs
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[0043] The present invention will be further described below in conjunction with specific examples.
[0044] Examples and comparative examples Utilize 2n pollen hybridization to breed lotus triploid
[0045] 1. Induction of 2n pollen
[0046] Be 3~3.5cm American lotus flower buds with the length as the processing object of the embodiment group of the present invention respectively, take the American lotus flower buds of 2.0cm as the comparative example group processing objects with the length, see figure 1 .
[0047] Except for the way of wrapping absorbent cotton and the amount of dripping liquid, if there is no special instruction, the other operations are the same:
[0048] Select flower buds with a length of 3 to 3.5 cm. The anthers are mainly milky white and slightly light yellow. This period is roughly the stage of primary spermatocytes. Gently push the outer petals of the flower buds ( figure 2 A), so that there is only one layer of 4-6 petals on the inner side to w...
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