Method for determining nervonic acid content by high performance liquid chromatography

A high-performance liquid chromatography and nervonic acid technology, which is applied in the field of using high-performance liquid chromatography to determine the content of nervonic acid, can solve the problems of long detection time, low efficiency and low reliability of detection results.

Pending Publication Date: 2021-04-30
云南自由贸易试验区睿之成医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using derivatization gas chromatography, it is first necessary to esterify the nervonic acid to determine the content of the esterified product. The pretreatment process of this method is cumbersome, and it is difficult to judge the end point of the esterification, and the reliability of the test result is not high.
There is also the prior art of using high-performance liquid chromatography to determine the content of nervonic acid, but there are still problems such as long detection time and low efficiency. Therefore, it is urgent to develop a method for determining the content of nervonic acid with simple sample pretreatment and high accuracy of measurement results. method

Method used

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  • Method for determining nervonic acid content by high performance liquid chromatography
  • Method for determining nervonic acid content by high performance liquid chromatography
  • Method for determining nervonic acid content by high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Accurately weigh about 15 mg of nervonic acid standard, put it in a 10 ml measuring bottle, add an appropriate amount of methanol and ultrasonically dissolve it for 2 minutes, and dilute to the mark as a control solution.

[0024] (2) Accurately weigh about 15 mg of nervonic acid raw material, put it in a 10 ml measuring bottle, add an appropriate amount of methanol and ultrasonically dissolve it for 2 minutes, and dilute to the mark as the test solution.

[0025] (3) The volume ratio of 0.01 mol / L potassium dihydrogen phosphate-methanol is 3:97 and the mobile equivalence is eluted, and the chromatographic column is C 18 , the detection wavelength is 200nm, the flow rate is 1.2ml / min, the injection volume is 10ul, and the column temperature is 30°C.

[0026] After testing, the peak time of nervonic acid in Example 1 was 7.9 minutes.

Embodiment 2

[0028] (1) Accurately weigh about 15 mg of nervonic acid standard, put it in a 10 ml measuring bottle, add an appropriate amount of methanol and ultrasonically dissolve it for 2 minutes, and dilute to the mark as a control solution.

[0029] (2) Accurately weigh about 15 mg of nervonic acid raw material, put it in a 10 ml measuring bottle, add an appropriate amount of methanol and ultrasonically dissolve it for 2 minutes, and dilute to the mark as the test solution.

[0030] (3) The volume ratio of 0.01 mol / L potassium dihydrogen phosphate-methanol is 3:97 as the flow equivalence for elution, and the chromatographic column is C 18 , the detection wavelength is 200nm, the flow rate is 1.0ml / min, the injection volume is 20ul, and the column temperature is 30°C.

[0031] After testing, the peak time of nervonic acid in Example 2 was 8.7 minutes.

Embodiment 3

[0033] (1) Accurately weigh about 15 mg of nervonic acid standard, put it in a 10 ml measuring bottle, add an appropriate amount of methanol and ultrasonically dissolve it for 2 minutes, and dilute to the mark as a control solution.

[0034] (2) Accurately weigh about 15 mg of nervonic acid raw material, put it in a 10 ml measuring bottle, add an appropriate amount of methanol and ultrasonically dissolve it for 2 minutes, and dilute to the mark as the test solution.

[0035] (3) The volume ratio of 0.01 mol / L potassium dihydrogen phosphate-methanol is 7:93 as the flow equivalence, and the chromatographic column is C 18 , the detection wavelength is 205nm, the flow rate is 0.8ml / min, the injection volume is 30ul, and the column temperature is 20°C.

[0036] After testing, the peak time of nervonic acid in Example 3 was 11.3 minutes.

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Abstract

The invention relates to the technical field of analytical chemistry, and particularly discloses a method for determining the content of nervonic acid by high performance liquid chromatography. The method comprises the following steps: taking a nervonic acid standard substance and a nervonic acid-containing sample to be detected, and carrying out ultrasonic treatment for 1-5 minutes by using methanol as a solvent, wherein the chromatographic conditions are as follows: a C18 chromatographic column is adopted, 0.01 mol / L monopotassium phosphate-methanol is taken as a mobile phase for isocratic elution. According to the invention, the parameters of the mobile phase are changed aiming at the prior art, octadecylsilane chemically bonded silica gel chromatographic column is adopted as a filler, the peak height and the peak area are not obviously influenced after testing, but the peak appearance time is greatly shortened, so the test speed is accelerated, and the efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for measuring nervonic acid content by high performance liquid chromatography. Background technique [0002] The chemical name of nervonic acid is cis-15-tetradecanoic acid, and its molecular formula is C 24 h 46 o 2 , the relative molecular weight is 366.6. At room temperature, nervonic acid is a white flaky crystal, soluble in alcohol but insoluble in water, with a melting point of 39-40°C. [0003] At present, derivatization gas chromatography is mostly used to determine the content of nervonic acid. Using derivatization gas chromatography, it is first necessary to esterify the nervonic acid and then measure the content of the esterified product. The pretreatment process of this method is cumbersome, and it is difficult to judge the end point of the esterification, and the reliability of the detection result is not high. There is also the prior art o...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor代玉玲王海云朱常成谢富兰李加靖席亮廖荣
Owner云南自由贸易试验区睿之成医药科技有限公司