Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Isomer of cholanic acid as well as detection method and application thereof

A technology for isomers and detection methods, which is applied in measurement devices, material separation, and analysis of materials, etc., can solve the problem that raw material impurities cannot be detected and evaluated accordingly.

Active Publication Date: 2021-05-11
SHANDONG INST FOR FOOD & DRUG CONTROL
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the current quality standards for Naoliqing preparations, the detection of pig bile substances usually uses TLC to identify the authenticity of pig gall medicinal materials, and to control the problem of replacing pig gall with beef and sheep gall, but for Naoliqing Whether there are quality risks introduced by composition changes in raw materials such as pig bile, pig bile powder, and pig bile paste in the preparation cannot be tested, and the impurities in the raw materials cannot be detected and evaluated accordingly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Isomer of cholanic acid as well as detection method and application thereof
  • Isomer of cholanic acid as well as detection method and application thereof
  • Isomer of cholanic acid as well as detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The detection of the isomer of embodiment 1 cholanic acid

[0034] Component analysis of pig bile, pig bile powder and pig bile paste

[0035] 1. Instrument, reagent and sample information

[0036] Instrument: Shimadzu LC-20A high performance liquid chromatograph, diode array detector;

[0037] Sartorius CP225D electronic balance

[0038] Reagents: Methanol and phosphoric acid are chromatographically pure, and the rest of the reagents are analytically pure.

[0039] Samples: 13 batches of pig bile powder, 2 batches of pig bile, and 1 batch of pig bile paste were collected through market collection, enterprise provision, and laboratory self-made.

[0040] Reference substances: Niuhuang hyodeoxycholic acid sodium salt (Zhenzhun Biology, batch number: ZS-20025), Niuhuang hyodeoxycholic acid (National Institute for Food and Drug Control, batch number: 111943-201802), taurine chenodeoxychole acid (National Institute for Food and Drug Control, batch number: 110846-201007)...

Embodiment 2

[0058] Example 2 Distribution of by-product 3β-type cholanic acid in pig gallbladder series products and Naoliqing preparations

[0059] The ratio of the peak area of ​​the by-product 3β-type cholanic acid to the sum of the peak areas of the two configurations of cholanic acid is used as the conversion rate evaluation index, that is, the conversion rate=A β / (A α +A β )×100%. In addition, three batches of high-temperature homemade pig gall powder were prepared (boiling and concentrating for 24 hours, drying at 121°C for 8 hours), and the average conversion rate was measured to be 10%, which was used as a limit.

[0060] The results of the analysis of pig bile series products showed that: ① the conversion rate of fresh pig bile collected in the market was lower than 1%; , the highest can reach 75%. It can be seen that the by-product 3β-cholanic acid widely exists in pig bile powder and pig bile ointment medicinal materials.

[0061] Through the investigation of enterprise ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an isomer of cholanic acid as well as a detection method and application thereof, a novel isomer 3 beta type cholanic acid of cholanic acid is found in a pig gall substance serving as a Naoliqing preparation raw material, and the byproduct widely exists in pig gall powder and pig bile paste medicinal materials and is transferred into a Naoliqing preparation along with the production feeding process, and the substance is used as an index for detecting the quality of the pig gall raw material, so that the quality risk caused by component change can be avoided.

Description

technical field [0001] The invention relates to an isomer of cholanic acid and its detection method and application, belonging to the field of analytical chemistry. Background technique [0002] The spatial configuration of the functional group plays a decisive role in the mechanism of action and clinical efficacy of the compound itself. The most typical case is the famous drug injury event in international history - the teratogenic effect of "reactin". Thalidomide, that is, "thalidomide", its two spatial configurations present completely different pharmacological and toxicological effects, the R type corresponds to a good sedative effect, and the S type has a strong teratogenic effect. Therefore, at the beginning of the 19th century, the research on the spatial configuration was not yet thorough, and the mixed use of the two isomers of thalidomide led to the birth of a large number of "seal children". Another typical drug - glycyrrhizic acid, as the current first-line drug...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 焦阳郭东晓尹雪于凤蕊徐兴燕林永强
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com