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Application of penthorum chinense pursh and compounds thereof in preparation of drugs for alleviating Alzheimer disease

A technology for Alzheimer's disease and the extract of C. chinensis, which is applied in the field of application of C. chinensis and its compounds in the preparation of drugs for alleviating Alzheimer's disease, and can solve problems such as untapped application potential

Pending Publication Date: 2021-05-18
THE AFFILIATED HOSPITAL OF SOUTHWEST MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the yellow grass has been used in folk prescriptions for protecting the liver, its mechanism of action and its application potential in the treatment of other diseases have yet to be explored

Method used

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  • Application of penthorum chinense pursh and compounds thereof in preparation of drugs for alleviating Alzheimer disease
  • Application of penthorum chinense pursh and compounds thereof in preparation of drugs for alleviating Alzheimer disease
  • Application of penthorum chinense pursh and compounds thereof in preparation of drugs for alleviating Alzheimer disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: Induction of autophagy by the total extract of C. chinensis

[0068] 1.1 Western blotting to detect the expression of autophagy-related proteins in Hela cells

[0069] Add 15-30 μg / mL drugs to the cultured Hela cells and incubate overnight: the extracts of the flowers of C. chinensis, the extracts of the leaves of C. sylvestris and the stems of C. sylvestris. Rapamycin (Rapa) was used as the autophagy positive control . After drug treatment, RIPA lysate (Cell Signaling Technologies Inc. Beverly, MA, USA) was added to each well at an amount of 70 μl / well to lyse HeLa cells, and Bradford reagent (Bio-Rad, Hercules, CA, USA) was used to measure protein concentration . Add an equal amount of protein to each well of the SDS-PAGE electrophoresis gel, electrophoresis at 300mA for 2 hours, and transfer the protein on the SDS-PAGE gel to the PVDF membrane. The PVDF membrane was blocked with 5% skimmed milk powder for 60 minutes and washed three times with TBST (1X)...

Embodiment 2

[0087] Example 2: Compounds TA, PG, TB, PG1 induce autophagy

[0088] 2.1 Determination of GFP-LC3 green fluorescent particles

[0089] The U87 cell line with GFP-LC3 stably transfected was transfected and established as in Example 1. After drug treatment, GFP-LC3 green fluorescent particles were detected.

[0090] 2.2 Western blotting

[0091] As described in Example 1, 4-8 μM compounds TA, PG, TB, and PG1 were used to treat HUVEC cells, the no-drug group was used as a blank control, and rapamycin (Rapa) was used as a positive control. After drug treatment, the HUVEC cells in each group were lysed by the above-mentioned method, SDS-PAGE protein electrophoresis, and the protein was transferred to PVDF membrane. After incubation with antibodies, ECL western blot detection solution (Invitrogen, Paisley, Scotland, UK) was used to expose and develop protein bands.

[0092] Result analysis: The autophagy activity of the above four compounds was analyzed by immunofluorescence tec...

Embodiment 3

[0131] Example 3: TA can reduce the level of oxidative stress in the aorta of the ApoE gene knockout atherosclerosis mouse model (ApoE-KO mice)

[0132] A 2-month-old ApoE gene knockout atherosclerosis mouse (ApoE-KO) model was established, and C57BL / 6J wild-type mice were used as the control group. Each group was given or not given intraperitoneal injection of TA (0.1mg / kg per day) for one month To evaluate the role of TA in the process of early aortic atherosclerosis in ApoE-KO knockout mice.

[0133] 3.1 Measurement of ROS in aortic vessels

[0134] 2',7'-dichlorofluorescin diacetate (2',7'-dichlorofluorescin diacetate, DCFH-DA) was used to measure the intracellular ROS content. Aortic vascular cells of ApoE-KO mice and C57BL / 6J mice were collected by Nylonsieve mesh combined with trypsin method. Add 10 μM DCFH-DA to the above cells, incubate at 37°C for 30 minutes, and then wash three times with serum-free medium. Intracellular esterase hydrolyzes DCFH-DA into DCFH so t...

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Abstract

The invention discloses application of an extract of a traditional Chinese medicine penthorum chinense pursh in preparation of drugs for alleviating Alzheimer disease. The extract plays a role by inducing autophagy, and four key compounds in the extract are disclosed. The extract of penthorum chinense pursh and the compounds thereof show an anti-atherosclerosis oxidative stress injury effect in-vivo and in-vitro experiments, have an inhibitory effect on the formation of beta-amyloid protein fibers and the expression of Tao protein which cause Alzheimer disease, and have potential application in alleviating Alzheimer disease.

Description

[0001] The application date is July 23, 2018, the application number is CN201810810099.X, and the name is "Application of Herba Grass and Its Compounds in the Preparation of Drugs for Treating Atherosclerosis and Alleviating Alzheimer's Disease" A divisional application of an invention patent application. technical field [0002] The present invention relates to the application of C. chinensis and its compounds in disease treatment or alleviation, in particular to the application in the preparation of medicines for treating atherosclerosis and alleviating Alzheimer's disease (Alzheimer disease, AD). Background technique [0003] It is a perennial herbaceous plant belonging to the saxifrage family and is a traditional Chinese medicinal material. It is recorded in ancient books that it has the effects of clearing away heat and detoxification, reducing yellowness and dampness, promoting blood circulation and dispelling blood stasis, diuresis and detumescence. figure 1 Shown are...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K31/7048A61P25/28
CPCA61K36/185A61K31/7048A61P25/28A61K2300/00
Inventor 孙晓磊黄锦伟罗婉君何延政余录
Owner THE AFFILIATED HOSPITAL OF SOUTHWEST MEDICAL UNIV
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