Simple and fast light microscope detection technology for blood and lymph of live shrimp and crab

Abstract

The prevent invention relates to a simple and quick detectino technique of shrimp-crab intravital blood lymph light microscope, including shrimp and crab living body blood-taking method: cleaning shrimp and crab body, disinfecting by using alcohol, using injector to needle into 7-10 mm of shrimp or crab body to draw blood; blood film preparation and staining method, using 0.05 ml of blood-lymph fluid, dropping on glass slide, adding 4% formaldehyde fixing fluid, drying, staining for 15-20 min. in Giemsa staining solution, washing film with water and making observation by using light microscope, covering with glass plate and making observation by using oil microscope. Said invention adopts meta-acid water flushing scheme to can quickly detect pathogen RLO (Rickettsia-like organism), therefore it possesses high practical value for culturing shrimp and crab.

Description

technical field [0001] The invention relates to a detection technology in aquaculture, in particular to a light microscope detection technology of shrimp and crab hemolymph. Background technique [0002] In the aquaculture industry, it is necessary to frequently check the health status of shrimp and crabs in order to prevent and control diseases, or use the test results as quality indicators for commercial shrimps and crabs. Among them, the light microscope detection technology of living shrimp and crab blood lymph is the most commonly used detection technology, including blood collection technology and smear staining technology. The existing blood collection technology is to drill a small hole in the heart area of ​​the carapace of shrimps and crabs, and insert the pericardial sinus with a straw to draw hemolymph. Another way of blood collection is to take blood from the feet. The former method of blood collection is lethal destruction; the latter is destructive destructi...

AI Technical Summary

Problems solved by technology

[0028] The hemolymph of shrimps and crabs is thicker and more likely to coagulate than the blood of higher animals. The dyeing methods of the above higher animal blood are often ineffective; the conventional Giemsa staining method is complex, cumbersome, time-consuming, and highly technical, requiring dyeing facilities There are more than 10 tanks and 5 to 8 kinds of medicines, which are not easy to promote and use at the grassroots level

At the same time, the xylene used in it is more harmful to the human body.

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